LOCUS       sequence-76703-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for TALEN with TALEColor Backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-NG(T)-Fok1
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             408..995
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
       61 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
      121 caggtcgacg gtctcgactc acgcctgagc aggtagtggc tattgcatcc aacggagggg
      181 gcagacccgc actggagtca atcgtggccc agctttcgag gccggacccc gcgctggccg
      241 cactcactaa tgatcatctt gtagcgctgg cctgcctcgg cggacgaccc gccttggatg
      301 cggtgaagaa ggggctcccg cacgcgcctg cattgattaa gcggaccaac agaaggattc
      361 ccgagaggac atcacatcga gtggcaggcc tgcagggaag tggttcccaa ctcgtgaaga
      421 gtgaacttga ggagaaaaag tcggagctgc ggcacaaatt gaaatacgta ccgcatgaat
      481 acatcgaact tatcgaaatt gctaggaact cgactcaaga cagaatcctt gagatgaagg
      541 taatggagtt ctttatgaag gtttatggat accgagggaa gcatctcggt ggatcacgaa
      601 aacccgacgg agcaatctat acggtgggga gcccgattga ttacggagtg atcgtcgaca
      661 cgaaagccta cagcggtggg tacaatcttc ccatcgggca ggcagatgag atgcaacgtt
      721 atgtcgaaga aaatcagacc aggaacaaac acatcaatcc aaatgagtgg tggaaagtgt
      781 atccttcatc agtgaccgag tttaagtttt tgtttgtctc tgggcatttc aaaggcaact
      841 ataaggccca gctcacacgg ttgaatcaca ttacgaactg caatggtgcg gttttgtccg
      901 tagaggaact gctcattggt ggagaaatga tcaaagcggg aactctgaca ctggaagaag
      961 tcagacgcaa gtttaacaat ggcgagatca atttccg
//
