LOCUS       sequence-76708-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL80012
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(165..269)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     337..355
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(393..415)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     515..534
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     728..750
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     742..759
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     743..759
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
       61 ctgtacccca aaaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
      121 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatactctt cctttttcaa
      181 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
      241 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
      301 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
      361 cgactcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagatg
      421 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
      481 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
      541 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
      601 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      661 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      721 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattcgagct cggtaccctt
      781 tgggcccagg aagttcggcg ttgctgggta ttgcccccat gcccccgctt atttcatttg
      841 gttcggcatc accttcagct ctcattgact cctggatttc agctaggcgt ttagctttct
      901 ttgcggcctg g
//
