LOCUS       sequence-76710-e        9081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A backbone for adenovirus creation by the AdEasy method, similar to 
            AdTrackCMV (Addgene Plasmid # 16405), except it co-expressed mRFP 
            instead of eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9081)
  TITLE     Bamburg lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: RedTrackCMV
FEATURES             Location/Qualifiers
     source          1..9081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_signal     198..348
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     polyA_signal    491..612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(760..1437)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(760..1437)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(822..845)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1020..1039)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1289..1307
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(1480..1683)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        1684..1987
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    2046..2167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(2445..2648)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6307..6324)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6478..7066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6558..6577)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             7873..8667
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7873..8667
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7927..7946)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8537..8556
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8777..8798)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8989..9008
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttaattaatc ccttccagct ctctgcccct tttggattga agccaatatg ataatgaggg
       61 ggtggagttt gtgacgtggc gcgggcgtgg gaacggggcg ggtgacgtag tagtgtggcg
      121 gaagtgtgat gttgcaagtg tggcggaaca catgtaagcg acggatgtgg caaaagtgac
      181 gtttttggtg tgcgccggtg tacacaggaa gtgacaattt tcgcgcggtt ttaggcggat
      241 gttgtagtaa atttgggcgt aaccgagtaa gatttggcca ttttcgcggg aaaactgaat
      301 aagaggaagt gaaatctgaa taattttgtg ttactcatag cgcgtaatat ttgtctaggg
      361 ccgcggggac tttgaccgtt tacgtggaga ctcgcccagg tgtttttctc aggtgttttc
      421 cgcgttccgg gtcaaagttg gcgttttatt attatagtca gtcgagtcta gcaagctagc
      481 ttgatcgcgt taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
      541 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      601 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
      661 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatctt
      721 gtggtatggc tgattatgat ctagagtcgc ggccaattct taggcgccgg tggagtggcg
      781 gccctcggcg cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc
      841 cagcttgatg tcggtcttgt aggcgccggg cagctgcacg ggcttcttgg ccatgtaggt
      901 ggtcttgacc tcggcgtcgt agtggccgcc gtccttcagc ttcagcctca tcttgatctc
      961 gcccttcagg gcgccgtcct cggggtacat ccgctcggtg gaggcctccc agcccatggt
     1021 cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta
     1081 gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc
     1141 gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg
     1201 gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac tggaactgag gggacaggat
     1261 gtcccaggcg aagggcaggg ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc
     1321 ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc
     1381 ctccatgcgc accttgaagc gcatgaactc cttgatgacg tcctcggagg aggccatcgg
     1441 atcccggtag cgctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc
     1501 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     1561 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     1621 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     1681 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     1741 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     1801 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     1861 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     1921 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     1981 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     2041 cgcgttaaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2101 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2161 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     2221 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagttatc
     2281 tagatccggt ggatctgagt ccggacttgt acagctcgtc catgccgaga gtgatcccgg
     2341 cggcggtcac gaactccaga tcggatatct tatctagaag cttaggctcg agcggccgcg
     2401 tcgacggtac cagatctcta gcggatctga cggttcacta aaccagctct gcttatatag
     2461 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     2521 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     2581 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     2641 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     2701 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     2761 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     2821 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     2881 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccaac
     2941 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attataaggg
     3001 tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg
     3061 ccgccgccat gagcaccaac tcgtttgatg gaagcattgt gagctcatat ttgacaacgc
     3121 gcatgccccc atgggccggg gtgcgtcaga atgtgatggg ctccagcatt gatggtcgcc
     3181 ccgtcctgcc cgcaaactct actaccttga cctacgagac cgtgtctgga acgccgttgg
     3241 agactgcagc ctccgccgcc gcttcagccg ctgcagccac cgcccgcggg attgtgactg
     3301 actttgcttt cctgagcccg cttgcaagca gtgcagcttc ccgttcatcc gcccgcgatg
     3361 acaagttgac ggctcttttg gcacaattgg attctttgac ccgggaactt aatgtcgttt
     3421 ctcagcagct gttggatctg cgccagcagg tttctgccct gaaggcttcc tcccctccca
     3481 atgcggttta aaacataaat aaaaaaccag actctgtttg gatttggatc aagcaagtgt
     3541 cttgctgtct ttatttaggg gttttgcgcg cgcggtaggc ccgggaccag cggtctcggt
     3601 cgttgagggt cctgtgtatt ttttccagga cgtggtaaag gtgactctgg atgttcagat
     3661 acatgggcat aagcccgtct ctggggtgga ggtagcacca ctgcagagct tcatgctgcg
     3721 gggtggtgtt gtagatgatc cagtcgtagc aggagcgctg ggcgtggtgc ctaaaaatgt
     3781 ctttcagtag caagctgatt gccaggggca ggcccttggt gtaagtgttt acaaagcggt
     3841 taagctggga tgggtgcata cgtggggata tgagatgcat cttggactgt atttttaggt
     3901 tggctatgtt cccagccata tccctccggg gattcatgtt gtgcagaacc accagcacag
     3961 tgtatccggt gcacttggga aatttgtcat gtagcttaga aggaaatgcg tggaagaact
     4021 tggagacgcc cttgtgacct ccaagatttt ccatgcattc gtccataatg atggcaatgg
     4081 gcccacgggc ggcggcctgg gcgaagatat ttctgggatc actaacgtca tagttgtgtt
     4141 ccaggatgag atcgtcatag gccattttta caaagcgcgg gcggagggtg ccagactgcg
     4201 gtataatggt tccatccggc ccaggggcgt agttaccctc acagatttgc atttcccacg
     4261 ctttgagttc agatgggggg atcatgtcta cctgcggggc gatgaagaaa acggtttccg
     4321 gggtagggga gatcagctgg gaagaaagca ggttcctgag cagctgcgac ttaccgcagc
     4381 cggtgggccc gtaaatcaca cctattaccg ggtgcaactg gtagttaaga gagctgcagc
     4441 tgccgtcatc cctgagcagg ggggccactt cgttaagcat gtccctgact cgcatgtttt
     4501 ccctgaccaa atccgccaga aggcgctcgc cgcccagcga tagcagttct tgcaaggaag
     4561 caaagttttt caacggtttg agaccgtccg ccgtaggcat gcttttgagc gtttgaccaa
     4621 gcagttccag gcggtcccac agctcggtca cctgctctac ggcatctcga tccagcatat
     4681 ctcctcgttt cgcgggttgg ggcggctttc gctgtacggc agtagtcggt gctcgtccag
     4741 acgggccagg gtcatgtctt tccacgggcg cagggtcctc gtcagcgtag tctgggtcac
     4801 ggtgaagggg tgcgctccgg gctgcgcgct ggccagggtg cgcttgaggc tggtcctgct
     4861 ggtgctgaag cgctgccggt cttcgccctg cgcgtcggcc aggtagcatt tgaccatggt
     4921 gtcatagtcc agcccctccg cggcgtggcc cttggcgcgc agcttgccct tggaggaggc
     4981 gccgcacgag gggcagtgca gacttttgag ggcgtagagc ttgggcgcga gaaataccga
     5041 ttccggggag taggcatccg cgccgcaggc cccgcagacg gtctcgcatt ccacgagcca
     5101 ggtgagctct ggccgttcgg ggtcaaaaac caggtttccc ccatgctttt tgatgcgttt
     5161 cttacctctg gtttccatga gccggtgtcc acgctcggtg acgaaaaggc tgtccgtgtc
     5221 cccgtataca gactgtttaa acgaattcta taaaatgcaa ggtgctgctc aaaaaatcag
     5281 gcaaagcctc gcgcaaaaaa gaaagcacat cgtagtcatg ctcatgcaga taaaggcagg
     5341 taagctccgg aaccaccaca gaaaaagaca ccatttttct ctcaaacatg tctgcgggtt
     5401 tctgcataaa cacaaaataa aataacaaaa aaacatttaa acattagaag cctgtcttac
     5461 aacaggaaaa acaaccctta taagcataag acggactacg gccatgccgg cgtgaccgta
     5521 aaaaaactgg tcaccgtgat taaaaagcac caccgacagc tcctcggtca tgtccggagt
     5581 cataatgtaa gactcggtaa acacatcagg ttgattcatc ggtcagtgct aaaaagcgac
     5641 cgaaatagcc cgggggaata catacccgca ggcgtagaga caacattaca gcccccatag
     5701 gaggtataac aaaattaata ggagagaaaa acacataaac acctgaaaaa ccctcctgcc
     5761 taggcaaaat agcaccctcc cgctccagaa caacatacag cgcttcacag cggcagccta
     5821 acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa aacaccactc gacacggcac
     5881 cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag agcgagtata tataggacta
     5941 aaaaatgacg taacggttaa agtccacaaa aaacacccag aaaaccgcac gcgaacctac
     6001 gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa tcgtcacttc cgttttccca
     6061 cgttacgtaa cttcccattt taagaaaact acaattccca acacatacaa gttactccgc
     6121 cctaaaacct acgtcacccg ccccgttccc acgccccgcg ccacgtcaca aactccaccc
     6181 cctcattatc atattggctt caatccaaaa taaggtatat tattgatgat ttaattaagg
     6241 atcccggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     6301 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6361 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6421 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6481 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6541 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6601 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6661 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6721 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6781 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6841 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6901 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6961 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7021 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7081 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7141 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7201 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7261 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7321 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7381 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7441 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7501 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgaaaaag gatcttcacc
     7561 tagatccttt tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc
     7621 agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc
     7681 agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa
     7741 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct
     7801 ttctcgccgc caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga
     7861 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     7921 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     7981 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     8041 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     8101 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     8161 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     8221 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     8281 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     8341 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     8401 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     8461 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     8521 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     8581 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     8641 tatcgccttc ttgacgagtt cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt
     8701 ttttaaccaa taggccgaaa tcggcaacat cccttataaa tcaaaagaat agaccgcgat
     8761 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     8821 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa
     8881 atcaagtttt ttgcggtcga ggtgccgtaa agctctaaat cggaacccta aagggagccc
     8941 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     9001 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     9061 acccgcgcgc ttaatgcgcc g
//
