LOCUS       sequence-76712-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL50014
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(283..301)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        369..473
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccaaccagct ccgaaacatc atcacaccct ccaggaacca ttgtggcccc catggctggt
       61 ttggtagtca aggtccttgt cgaaaacgaa gccaaagtag atcaaggtca acctatatta
      121 gtcctagagg caatgaagat ggagcacgtt gtgaaagcac catcctctgg aagcatacag
      181 gacctcaaag ttaaagcagg ccaacaggtt tcagatggca gtgctctatt caggatcaaa
      241 gggtaagctt tgagaccacg tggctcttca gtggacgaaa gggcctcgtg atacgcctat
      301 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
      361 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      421 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgcgct
      481 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
      541 tcatggcttg ttatgactgt ttttttttgg ggtacagtct atgcctcggg catccaagca
      601 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
      661 gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt
      721 atcgactcaa ctatca
//
