LOCUS       sequence-76714-e        7981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A backbone for adenovirus creation by the AdEasy method, similar to 
            pShuttle-CMV (Addgene #16403), except it uses a Neuron-specific 
            enolase (NSE) promoter instead of the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Mi J, Shaw AE, Pak CW, Walsh KP, Minamide LS, Bernstein BW, Kuhn TB,
            Bamburg JR
  TITLE     A genetically encoded reporter for real-time imaging of 
            cofilin-actin rods in living neurons.
  JOURNAL   PLoS One. 2013 Dec 31;8(12):e83609. doi: 
            10.1371/journal.pone.0083609.
  PUBMED    24391794
REFERENCE   2  (bases 1 to 7981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-31"; volume: "8(12):e83609. doi"; pages: " 
            10.1371/journal.pone.0083609"
COMMENT     Sequence Label: ShuttleNSE
FEATURES             Location/Qualifiers
     source          1..7981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_signal     202..352
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     polyA_signal    1740..1861
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1777..1796)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1831..1850
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5201..5218)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5372..5960)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5452..5471)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             6773..7567
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6773..7567
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6827..6846)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7437..7456
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7677..7698)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7889..7908
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttaattaann ntcccttcca gctctctgcc ccttttggat tgaagccaat atgataatga
       61 gggggtggag tttgtgacgt ggcgcggggc gtgggaacgg ggcgggtgac gtagtagtgt
      121 ggcggaagtg tgatgttgca agtgtggcgg aacacatgta agcgacggat gtggcaaaag
      181 tgacgttttt ggtgtgcgcc ggtgtacaca ggaagtgaca attttcgcgc ggttttaggc
      241 ggatgttgta gtaaatttgg gcgtaaccga gtaagatttg gccattttcg cgggaaaact
      301 gaataagagg aagtgaaatc tgaataattt tgtgttactc atagcgcgta atactgtaat
      361 cttaagggga cagtaaaggt gatggcagga aggcagcccc cggaggtcaa aggctgggca
      421 cgcgggagga gaggccagag tcagaggctg cgggtatctc agatatgaag gaaagatgag
      481 agaggctcag gaagaggtaa gaaaagacac aagagaccag agaagggaga agaattagag
      541 agggaggcag aggaccgctg tctctacaga catagctggt agagactggg aggaagggat
      601 gaaccctgag cgcatgaagg gaaggaggtg gctggtggta tatggaggat gtagctgggc
      661 cagggaaaag atcctgcact aaaaatctga agctaaaaat aacaggacac ggggtggaga
      721 ggcgaaagga gggcagattg aggcagagag actgagaggc ctggggatgt gggcattccg
      781 gtagggcaca cagttcactt gtcttctctt tttccaggag gccaaagatg ctgacctcaa
      841 gaactcataa taccccagtg gggaccaccg cattcatagc cctgttacaa gaagtgggag
      901 atgttccttt ttgtcccaga ctggaaatcc attacatccc gaggctcagg ttctgtggtg
      961 gtcatctctg tgtggcttgt tctgtgggcc tacctaaagt cctaagcaca gctctcaagc
     1021 agatccgagg cgactaagat gctagtaggg gttgtctgga gagaagagcc gaggaggtgg
     1081 gctgtgatgg atcagttcag ctttcaaata aaaaggcgtt tttatattct gtgtcgagtt
     1141 cgtgaacccc tgtggtgggc ttctccatct gtctgggtta gtacctgcca ctatactgga
     1201 ataaggagac gcctgcttcc ctcgagttgg ctggacaagg ttatgagcat ccgtgtactt
     1261 atggggttgc cagcttggtc ctggatcgcc cgggcccttc ccccacccgt tcggttcccc
     1321 accaccaccc gcgctcgtac gtgcgtctcc gcctgcagct cttgactcat cggggccccc
     1381 gggtcacatg cgctcgctcg gctctatagg cgccgccccc tgcccacccc ccgcccgcgc
     1441 tgggagccgc agccgccgcc actcctgctc tctctgcgcc gccgccgtca ccaccgccac
     1501 cgccaccggc tgagtctgca gtcctccgaa acgggccctc tagactcgag cggccgccac
     1561 tgtgctggat atctgcagaa ttccaccaca ctggactagt ggatccgagc tcggtaccaa
     1621 gcttaagttt ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     1681 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     1741 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     1801 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     1861 aatcgacaga tctnnntaag ggtgggaaag aatatataag gtgggggtct tatgtagttt
     1921 tgtatctgtt ttgcagcagc cgccgccgcc atgagcacca actcgtttga tggaagcatt
     1981 gtgagctcat atttgacaac gcgcatgccc ccatgggccg gggtgcgtca gaatgtgatg
     2041 ggctccagca ttgatggtcg ccccgtcctg cccgcaaact ctactacctt gacctacgag
     2101 accgtgtctg gaacgccgtt ggagactgca gcctccgccg ccgcttcagc cgctgcagcc
     2161 accgcccgcg ggattgtgac tgactttgct ttcctgagcc cgcttgcaag cagtgcagct
     2221 tcccgttcat ccgcccgcga tgacaagttg acggctcttt tggcacaatt ggattctttg
     2281 acccgggaac ttaatgtcgt ttctcagcag ctgttggatc tgcgccagca ggtttctgcc
     2341 ctgaaggctt cctcccctcc caatgcggtt taaaacataa ataaaaaacc agactctgtt
     2401 tggatttgga tcaagcaagt gtcttgctgt ctttatttag gggttttgcg cgcgcggtag
     2461 gcccgggacc agcggtctcg gtcgttgagg gtcctgtgta ttttttccag gacgtggtaa
     2521 aggtgactct ggatgttcag atacatgggc ataagcccgt ctctggggtg gaggtagcac
     2581 cactgcagag cttcatgctg cggggtggtg ttgtagatga tccagtcgta gcaggagcgc
     2641 tgggcgtggt gcctaaaaat gtctttcagt agcaagctga ttgccagggg caggcccttg
     2701 gtgtaagtgt ttacaaagcg gttaagctgg gatgggtgca tacgtgggga tatgagatgc
     2761 atcttggact gtatttttag gttggctatg ttcccagcca tatccctccg gggattcatg
     2821 ttgtgcagaa ccaccagcac agtgtatccg gtgcacttgg gaaatttgtc atgtagctta
     2881 gaaggaaatg cgtggaagaa cttggagacg cccttgtgac ctccaagatt ttccatgcat
     2941 tcgtccataa tgatggcaat gggcccacgg gcggcggcct gggcgaagat atttctggga
     3001 tcactaacgt catagttgtg ttccaggatg agatcgtcat aggccatttt tacaaagcgc
     3061 gggcggaggg tgccagactg cggtataatg gttccatccg gcccaggggc gtagttaccc
     3121 tcacagattt gcatttccca cgctttgagt tcagatgggg ggatcatgtc tacctgcggg
     3181 gcgatgaaga aaacggtttc cggggtaggg gagatcagct gggaagaaag caggttcctg
     3241 agcagctgcg acttaccgca gccggtgggc ccgtaaatca cacctattac cgggtgcaac
     3301 tggtagttaa gagagctgca gctgccgtca tccctgagca ggggggccac ttcgttaagc
     3361 atgtccctga ctcgcatgtt ttccctgacc aaatccgcca gaaggcgctc gccgcccagc
     3421 gatagcagtt cttgcaagga agcaaagttt ttcaacggtt tgagaccgtc cgccgtaggc
     3481 atgcttttga gcgtttgacc aagcagttcc aggcggtccc acagctcggt cacctgctct
     3541 acggcatctc gatccagcat atctcctcgt ttcgcgggtt ggggcggctt tcgctgtacg
     3601 gcagtagtcg gtgctcgtcc agacgggcca gggtcatgtc tttccacggg cgcagggtcc
     3661 tcgtcagcgt agtctgggtc acggtgaagg ggtgcgctcc gggctgcgcg ctggccaggg
     3721 tgcgcttgag gctggtcctg ctggtgctga agcgctgccg gtcttcgccc tgcgcgtcgg
     3781 ccaggtagca tttgaccatg gtgtcatagt ccagcccctc cgcggcgtgg cccttggcgc
     3841 gcagcttgcc cttggaggag gcgccgcacg aggggcagtg cagacttttg agggcgtaga
     3901 gcttgggcgc gagaaatacc gattccgggg agtaggcatc cgcgccgcag gccccgcaga
     3961 cggtctcgca ttccacgagc caggtgagct ctggccgttc ggggtcaaaa accaggtttc
     4021 ccccatgctt tttgatgcgt ttcttacctc tggtttccat gagccggtgt ccacgctcgg
     4081 tgacgaaaag gctgtccgtg tccccgtata cagactnnng tttaaacgaa ttcnnntata
     4141 aaatgcaagg tgctgctcaa aaaatcaggc aaagcctcgc gcaaaaaaga aagcacatcg
     4201 tagtcatgct catgcagata aaggcaggta agctccggaa ccaccacaga aaaagacacc
     4261 atttttctct caaacatgtc tgcgggtttc tgcataaaca caaaataaaa taacaaaaaa
     4321 acatttaaac attagaagcc tgtcttacaa caggaaaaac aacccttata agcataagac
     4381 ggactacggc catgccggcg tgaccgtaaa aaaactggtc accgtgatta aaaagcacca
     4441 ccgacagctc ctcggtcatg tccggagtca taatgtaaga ctcggtaaac acatcaggtt
     4501 gattcatcgg tcagtgctaa aaagcgaccg aaatagcccg ggggaataca tacccgcagg
     4561 cgtagagaca acattacagc ccccatagga ggtataacaa aattaatagg agagaaaaac
     4621 acataaacac ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg ctccagaaca
     4681 acatacagcg cttcacagcg gcagcctaac agtcagcctt accagtaaaa aagaaaacct
     4741 attaaaaaaa caccactcga cacggcacca gctcaatcag tcacagtgta aaaaagggcc
     4801 aagtgcagag cgagtatata taggactaaa aaatgacgta acggttaaag tccacaaaaa
     4861 acacccagaa aaccgcacgc gaacctacgc ccagaaacga aagccaaaaa acccacaact
     4921 tcctcaaatc gtcacttccg ttttcccacg ttacgtaact tcccatttta agaaaactac
     4981 aattcccaac acatacaagt tactccgccc taaaacctac gtcacccgcc ccgttcccac
     5041 gccccgcgcc acgtcacaaa ctccaccccc tcattatcat attggcttca atccaaaata
     5101 aggtatatta ttgatgatnn nttaattaag gatccnnncg gtgtgaaata ccgcacagat
     5161 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     5221 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5281 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5341 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5401 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5461 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5521 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5581 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5641 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5701 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5761 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     5821 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5881 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5941 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6001 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6061 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6121 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6181 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6241 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6301 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6361 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6421 gtttgcgcaa cgttgttgnn nnnnaaaaag gatcttcacc tagatccttt tcacgtagaa
     6481 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac
     6541 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata
     6601 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
     6661 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc caaggatctg
     6721 atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     6781 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     6841 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     6901 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     6961 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     7021 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     7081 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     7141 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     7201 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     7261 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     7321 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     7381 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     7441 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     7501 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     7561 cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     7621 tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt gttgttccag
     7681 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     7741 tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt ttgcggtcga
     7801 ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     7861 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     7921 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc
     7981 g
//
