LOCUS       sequence-76715-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian N. meningitidis Cas9 expression, human optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-NMcas
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
       61 ggaatcgaaa tctcgtagca cgtgctattc ctttgccctc ggacgagtgc tggggcgtcg
      121 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
      181 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
      241 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
      301 accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg
      361 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
      421 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
      481 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
      541 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
      601 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
      661 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
      721 cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac
      781 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat
      841 gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa tcgggagcgc
      901 ggccgatgca aagtgccgat
//
