LOCUS       sequence-76716-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH44222
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(125..143)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        211..315
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acaagagcgt acggttcaat ccctgcctcc cctgtaaaac taccctttga aaacctctct
       61 ttcttaatct tttctttgta attccaatgt gagaccacga agtggctctt cagtggacga
      121 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
      181 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      241 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      301 tgaaaaagga agagtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
      361 ggtaacggcg cagtggcggt tttcatggct tgttatgact gttttttttt ggggtacagt
      421 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
      481 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga
      541 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
      601 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
      661 tgaagccaca cagcgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
      721 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
      781 ttctccgcgc tgtagaagtc accattgttg tgcacgacga
//
