LOCUS       sequence-76718-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH71292
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..76
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
       61 aggcgtatca cgaggccctt tcgtccactg aagagccact tcgtggtctc acattgcgtt
      121 tacttactta gctataaaat gttttttttt tttttaattt caaagaaact atattttttt
      181 taaagatgct tattacacag tggtatcgtc cctttatata atgataaggt tttagacgtt
      241 ttttaaaatc taacggatag aaacaaaaga agcctcatct tagatgaagg gcgaccattg
      301 gatttggttg ttgatatatt acaaagttta atggccacat gaaaggtaag aaattggagc
      361 taagtgcctt tttttaaaaa atattttacg ccctctattt aaaaaaaaaa tgtctctatt
      421 ttttttattc tgtttcaaaa aaatgatctc tttccttttc tgataacact ttaattttaa
      481 tttttcacgt gacatattta agaccacaag attgaaggac attttgatat atttgacata
      541 actttaattt aaaatcacaa aatcaaaaag ttttctttcg tttctcaaac tacgtttcaa
      601 gtcaaactag atcatccttt ttaaccagag tgagtgagta atagacaaga atgaaggtgc
      661 cacgtgtcgg aatatggtga atcttatctc atccaagata tgaatgtgtg atagtgcatt
      721 gttggttaat ttttccatgt atttaagagt gtagtttagc ctcatattgc agtctttttt
      781 ggattggact taagacagac cattccttgc tgcagaaggt ccatattttt ctggatgtat
      841 cattttcagt tttgggcact actagttggt tgaattttta gaaaaaattt aacacataaa
      901 tacggacaat atacatttta atattttata tactttgttt gcttttg
//
