LOCUS       sequence-76722-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH71301
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..75
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
       61 ggcgtatcac gaggcccttt cgtccactga agagccactt cgtggtctca cattttgctt
      121 cttctctctt tttttggttc aatatgaacc ttttgatgtc cactatcctt ttatataatg
      181 aaatgataag gtttttgata tgttatgtgg ttcttgataa cattatacaa ttacttaata
      241 tctacatatg aaaggttgga atttttttta agtcaccaca atagaggtga cacgtgtaag
      301 cacctcgtta atcttatctc atccaagatg ggggtaggaa gaggatatga atgtatgatt
      361 gaggttggtt ttgagttttt tttttttttt tttttgagtc cttcaacttg ttattttaat
      421 ttttttttgg tgggggagga ggggggttga aatatttatc atatagtagt ccaaagtaaa
      481 ttgatagcta gagtacttgt ttgcttgctt atattgtcct caactttatg taataccatg
      541 attccaactt agacactctt ttaagttgta attttcatta ttttcttttt ttagagtttt
      601 atgttgaatt cgcataattt tcaatcggat aatacaagaa aaataatatt ttagtataat
      661 tttatacatg aaatttcggg aaggtaggat atacggattg tttgtcggat cagagacttt
      721 actcgtacct ttgtaactgt tgatcccaaa tacagatagt gactccagag ttatatatta
      781 tacctataga gactaatatg atttagtgtt attaaaatta gtattactaa ttaattgtaa
      841 tgccccatga attctcctga
//
