LOCUS       sequence-76724-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH71342
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..70
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
       61 atcacgaggc cctttcgtcc actgaagagc cacttcgtgg tctcacattg aaatttgaaa
      121 aaggtgatga ttctagggtt atgaaatttg atgattgatg aaggggatgg tggtggctat
      181 ttatagttgg tgaatttggg cgtcgaggat ttcgtgggcg gtttgaaatg aggcggggtt
      241 aaaatcatgg ccgctaaatt ttgggaaatt tattgataat cggacggata agaatgtcga
      301 tccacgtgaa tggagaaaac tgaatataag ccgttgatct gaatggagat ctacggtgtc
      361 gagtgcttct ttgtgtttgg acacggattg tgctttttca gtgagagaaa aagagggggt
      421 tacgagaatc taaattattt gtgggccgcc aaataaagaa aagatagtct caaattattt
      481 atttagtcct catctatttt atcagacgtg tattaaaatt agaatatcta aatttaaata
      541 gtattaagtt ttgaatatta aataaattaa aattatttga ttcttaattt ttgaatatat
      601 gactcgtctt ttatttatat taaaacatat atgtagctat atcaataata tgttttcaac
      661 atgtatatga tgtttgatgt gtgattttgg gtgtccatta aaaagatggt atatagattc
      721 atccgaataa ttaatttaga tatttaaatt agggctagta catattagac acctatacct
      781 cttaaaattg atatatattt tgatattttt tgacaatcaa tcaaatatat gtgatgtttg
      841 taatgtactc gctatgacaa ggcaaattga tgaattaaat aatacacgta acatttgaat
      901 tcaaaatttt acttaaaaat gatattctcc tgagaccaca ga
//
