LOCUS       sequence-76725-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses split fluorescence protein to measure caspase-2 
            dimerization in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Bouchier-Hayes L, Oberst A, McStay GP, Connell S, Tait SW, Dillon 
            CP, Flanagan JM, Beere HM, Green DR
  TITLE     Characterization of cytoplasmic caspase-2 activation by induced 
            proximity.
  JOURNAL   Mol Cell. 2009 Sep 24;35(6):830-40. doi: 
            10.1016/j.molcel.2009.07.023.
  PUBMED    19782032
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.07"; journalName: "Mol Cell"; date: 
            "2009-09-24- 24"; volume: "35"; issue: "6"; pages: "830-40"
COMMENT     Sequence Label: pBiFC-flag-hCaspase 2 CARD-VN173
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      361..370
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(412..433)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gaaactctaa aaaagaaccg agtggtgcta gccaaacagc tgttgttgag cgaattgtta
       61 gaacatcttc tggagaagga catcatcacc ttggaaatga gggagctcat ccaggccaaa
      121 gtgggcagtt tcagccagaa tgtggaactc ctcaacttgc tgcctaagag gggtccccaa
      181 gcttttgatg ccttctgtga agcactgagg gagaccaagc aaggccacct ggaggatatg
      241 ttgctcacca ccctttctgg gcttcagcat gtactcccac cgttgagctg tgactacgac
      301 ttgagtggga attcatcgat agatctgata tcggtaccag tcgactctag aagatccatc
      361 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      421 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      481 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
      541 cccaccctcg tgaccaccct gggctacggc ctgcagtgct tcgcccgcta ccccgaccac
      601 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      661 atcttcttca aggacgacgg caactacaga cccgcgccga ggtgaagttc gagggcgaca
      721 ccctggtgaa ccgcatcgag ctgaaggcat cgacttcaag gaggacggca acatcctggg
      781 gcacaagctg gagtacaact acacagccac aacgtctat
//
