LOCUS       sequence-76727-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Galactose-inducible expression of ER-membrane S. pyogenes SrtA in 
            yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Strijbis K, Spooner E, Ploegh HL
  TITLE     Protein ligation in living cells using sortase.
  JOURNAL   Traffic. 2012 Jun;13(6):780-9. doi: 
            10.1111/j.1600-0854.2012.01345.x. Epub 2012 Mar 23.
  PUBMED    22348280
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2012.01345.x"; journalName: "Traffic"; date: 
            "2012-06"; volume: "13"; issue: "6"; pages: "780-9"
COMMENT     Sequence Label: pRS303-pGAL-HA-TMSec66-SrtA (pKS105)
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(667..693)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(723..746)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga gctaggtaga
       61 tacttggtta taagaatgat taaaagcttt caatacatcg gcgggcgctt tatcaaagtc
      121 gtattctgtt tttagttctc ctttgacaat aatacgttct gttgcttcga tatctgtaca
      181 agtcactaaa gtcacttctt tgagaccagc tgtatcatcg ataacatcaa cgcgttcagg
      241 agctaccgtg aaaacatctt ttatgatgta ttcgtaaatt ttttctttat ctgttaaata
      301 gatggacatc ccattttgtg ctctttcaag cggcgaaaag agcatttgag atgaacctgt
      361 aattccaaaa atatgatgac tggcaagaga ataattattt tctcctccca taacttgttc
      421 ttctttcatc gttcctgcgc cataaataag ctcagtattt cctaaacctt taaaaattgg
      481 taaattaatg ccaagctctg gtatggcaat gccccctata acaggaagtt gctgagccgc
      541 catttgtgct tgcaagacac tctctgtact aactggctcg gatccgcttg aagcaaacat
      601 cacaagggac accaccagaa taaagacata tatgagtggg gtataaacgg agattgattt
      661 cgtctctgcg tagtctggta cgtcgtatgg gtacattcta gaatccgggg ttttttctcc
      721 ttgacgttaa agtatagagg tatattaaca attttttgtt gatactttta ttacatttga
      781 ataagaagta atacaaaccg aaaatgttga aagtattagt taaagtggtt atgcagtttt
      841 tgcatttata tatctgttaa tagatcaaaa atcatcgctt cgctgattaa ttaccccaga
      901 aataaggcta aaaaactaat cgcattatca tcctatggtt gttaatttga ttcgttcatt
      961 tgaa
//
