LOCUS       sequence-76731-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hygromycin resistant; expresses luxAB-DAS+4 under the control of a 
            promoter containing PtetO-4C5G; constitutively expresses reverse 
            TetR (tetR38); replicates episomally.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Kim JH, O'Brien KM, Sharma R, Boshoff HI, Rehren G, Chakraborty S, 
            Wallach JB, Monteleone M, Wilson DJ, Aldrich CC, Barry CE 3rd, Rhee 
            KY, Ehrt S, Schnappinger D
  TITLE     A genetic strategy to identify targets for the development of drugs 
            that prevent bacterial persistence.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Nov 4.
  PUBMED    24191058
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Nov 4."
COMMENT     Sequence Label: pGMEH-T38M-750-luxAB-DAS+4
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cttgatatcg aattcctgca gcccggggga tccatttaaa tcagagcttt cgcgagctcg
       61 agatccggct gtttttgttc ctgcaatgac cattgctgag gagttcccat gaaaatcaag
      121 tcatcgattt ctgtgctcgc tgcgatattt tcctgcttaa ccgctgaaag tacgtccata
      181 taaatgcctc tattagttag cgctatcgcg cgaaaagaat ggtgatataa ggggcatcgc
      241 tgccccttca gcatcagtta aacgactagt tattagctgg cgtccgcgta gttctcggag
      301 tagttctcgt cgttggcggc cgagtggtat ttgacgatgt tggcgtttac cacatcgata
      361 accgctcttt gctgcgcttt atcttccatc gactcaaaag acatcaatag gtccgcggca
      421 ccacaacact caatcgcaac tcgcgctgcc tgagtacttt cttcataagt accgatggca
      481 ttttctgaca acagctctcc cattttttgc tcaaagtcgg tatttgagta agattcacgg
      541 acaaactctt ccaaatacac gcgagcttct gcccttgctg cttcaccatc tacattttgg
      601 ttgaccagca gcgtcagctt gtgtcgaacc tgactaacat cgacaccatg tgcctgagca
      661 acttcgtggt acaaaccggc gtattctttt ctttgagcgt ttgagtcgtc ccatctaaac
      721 acgagtggaa gccctaactt agccgcccat tcaaccactt ctttgctcgt cgcattcaca
      781 aattgcgcag gaccgccttc agtgaacgc
//
