LOCUS       sequence-76736-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 hCas9 module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V
  TITLE     Plant genome editing made easy: targeted mutagenesis in model and 
            crop plants using the CRISPR/Cas system.
  JOURNAL   Plant Methods. 2013 Oct 11;9(1):39.
  PUBMED    24112467
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2013-10-11"; volume: "9(1)"; pages: "39"
COMMENT     Sequence Label: pICH47742::2x35S-5'UTR-hCas9(STOP)-NOST
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(53..305)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(321..341)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgtcaaacac tgatagttta aaccacttcg tgcagaagac aatagtagcg tcgatctagt
       61 aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct
      121 atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac
      181 gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata
      241 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
      301 cgatctgctt gacaagctca caccttcctc ttcttcttgg ggtcagccct gctgtctcca
      361 ccgagctgag agaggtcgat tcttgtttca tagagccccg taattgactg atgaatcagt
      421 gtggcgtcca ggacctcctt tgtagaggtg taccgctttc tgtctatggt ggtgtcgaag
      481 tacttgaagg ctgcaggcgc gcccaagttg gtcagagtaa acaagtggat aatgttttct
      541 gcctgctccc tgatgggctt atccctgtgc ttattgtaag cagaaagcac cttatcgagg
      601 ttagcgtcgg cgaggatcac tcttttggag aattcgctta tttgctcgat gatctcatca
      661 aggtagtgtt tgtgttgttc cacgaacagc tgcttctgct cattatcttc gggagatcct
      721 ttgagctttt catagtggct ggccagatac aagaaattaa cgtatttaga gggcagtgcc
      781 agctcgttac ctttctgcag ctcgcccgca ctagcgagca ttcgtttccg gccgttttca
      841 agctcaaaga gagagtactt gggaagctta atgatgaggt cttttttgac ctctttatat
      901 cctttcgcct cgagaaagtc gatggggttt ttt
//
