LOCUS       sequence-76737-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 6, LacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_6_XcmI-LacZ
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    226..247
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(262..267,268..285,286..292)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    300..316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     305..327
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     324..340
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     324..340
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             336..719
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGREFTSDIEFPR
                     PPWRPGACDVGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
                     RTDRPSQQLRSLNGEWTRPVAAH"
     promoter        358..376
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      470..479
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      474..483
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(513..532)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(514..532)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(539..556)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(539..555)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(548..570)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        complement(757..775)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 cctgaagttc tcaggatcgg tcgagagcct tacttagtta cccgggagga tgagggtctc
       61 aaactggtac cagcggtggc gtggcgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      241 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      301 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctatt
      361 taggtgacac tatagaatac tcaagctatg catccaacgc gttgggagct ctcccatatg
      421 gtcgacctgc aggcggccgc gaattcacta gtgatatcga attcccgcgg ccgccatggc
      481 ggccgggagc atgcgacgtc gggcccaatt cgccctatag tgagtcgtat tacaattcac
      541 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      601 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      661 cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag
      721 cgcgccacgc caccgctgga tccgcctgat gagacctcta tagtgtcacc taaatcaagc
      781 ttgctccgac gtccccaggc agaatggcgg ttccctaaac gagcattgct tatatagacc
      841 tcccattagg cacgcctacc gcccatttac gtcaatggaa cgcccatttg cgtcattgcc
      901 cctccccatt gac
//
