LOCUS       sequence-76741-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 hCas9 module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V
  TITLE     Plant genome editing made easy: targeted mutagenesis in model and 
            crop plants using the CRISPR/Cas system.
  JOURNAL   Plant Methods. 2013 Oct 11;9(1):39.
  PUBMED    24112467
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2013-10-11"; volume: "9(1)"; pages: "39"
COMMENT     Sequence Label: pICH47742::2x35S-5'UTR-hCas9(STOP)-NOST
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(86..97)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgttgtgtga tcagtttggc gttcagcagc tgccgccaat aatttttcat tttcttgaca
       61 acttcttctg aggggacgtt atcactcttc cctctatttt tatcggatct tgtcaacact
      121 ttattatcaa tagaatcatc tttgagaaaa gactggggca cgatatgatc cacgtcgtag
      181 tcggagagcc gattgatgtc cagttcctga tccacgtaca tgtccctgcc gttctgcagg
      241 tagtacaggt agagcttctc attctgaagc tgggtgtttt caactgggtg ttccttaagg
      301 atttgggacc ccagttcttt tataccctct tcaatcctct tcatcctttc cctactgttc
      361 ttctgtccct tctgggtagt ttggttctct cgggccatct cgataacgat attctcgggc
      421 ttatgccttc ccattacttt gacgagttca tccacgacct taacggtctg cagtattccc
      481 tttttgatag ctgggctacc tgcaagatta gcgatgtgct cgtggagact gtccccctgg
      541 ccagaaactt gtgctttctg gatgtcctcc ttaaaggtga gagagtcatc atggatcaac
      601 tgcatgaagt tccggttggc aaatccatcg gacttaagaa aatccaggat tgtctttcca
      661 ctctgcttgt ctcggatccc attgatcagt tttcttgaca gccgccccca tcctgtatat
      721 cggcgcctct tgagctgttt catgactttg tcgtcgaaga gatgagcgta agttttcaag
      781 cgttcttcaa tcatctccct atcttcaaac aacgtaaggg tgaggacaat gtcctcaaga
      841 atgtcctcgt tctcctcatt gtccaggaag tccttgtctt taatgatttt c
//
