LOCUS       sequence-76748-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 
            promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUN6A11
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(311..331)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(338..403)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 aagaatcgtc aaccttcgcc atctcgttgg agaaaatctc ctgcaggtag cagatgcgat
       61 tcttcctgcg cgtgtaccgc ctgcgggcgg tgcgcttgag ccgcgtagcc tcagccgtct
      121 cgccgctgtc gaacaggaga gcgccaatga gattcttctt gatggaatgc cgatcggtgt
      181 tgcccaggac cttgaacttc tttgagggca ccttgtactc gtcggtgatc acggcccagc
      241 caacagagtt agtcccaatg gcgaggccga tcgagtactt cttgtcagca gctggcaccc
      301 cgtggatgcc aaccttcctc ttcttcttcg gagccatctt gtcatcatca tccttgtaat
      361 caatgtcgtg gtccttgtaa tccccgtcgt ggtccttgta atccatctag agcggccgcg
      421 gccatacaag tattggggat cccccgggct gcagaagtaa caccaaacaa cagggtgagc
      481 atcgacaaaa gaaacagtac caagcaaata aatagcgtat gaaggcaggg ctaaaaaaat
      541 ccacatatag ctgctgcata tgccatcatc caagtatatc aagatcaaaa taattataaa
      601 acatacttgt ttattataat agataggtac tcaaggttag agcatatgaa tagatgctgc
      661 atatgccatc atgtatatgc atcagtaaaa cccacatcaa catgtatacc tatcctagat
      721 cgatatttcc atccatctta aactcgtaac tatgaagatg tatgacacac acatacagtt
      781 ccaaaattaa taaatacacc aggtagtttg aaacagtatt ctactccgat ctagaacgaa
      841 tgaacgaccg cccaaccaca ccacatcatc acaaccaagc gaacaaaaag catctctgta
      901 tatgcatcag taaaacccgc atcaacatgt atacctatcc tagatcgata tttccatcca
      961 tcatcttcaa
//
