LOCUS       sequence-76769-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of C-terminal yEGFP tagged gene 
            sequences together with 4 copies of the core DSR motif into the S. 
            pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-cyEGFP-4XDSR
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(85..801)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(88..801)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 cttcgtatat aataccatat acgaagttat cccggggttt aaagttctgt ttaagaatgg
       61 gtttaatacg gagtttaaag atctttattt gtacaattca tccataccat gggtaatacc
      121 agcagcagta acaaattcta acaagaccat gtggtctctc ttttcgtttg gatctttgga
      181 taaggcagat tgagtggata agtaatggtt gtctggtaac aagactggac catcaccaat
      241 tggagtattt tgttgataat ggtcagctaa ttgaacagaa ccatcttcaa tgttgtgtct
      301 aattttgaag ttaactttga taccattctt ttgtttgtca gccatgatgt aaacattgtg
      361 agagttatag ttgtattcca atttgtgacc taaaatgtta ccatcttctt taaaatcaat
      421 accttttaat tcgattctat taactaaggt atcaccttca aacttgactt cagctctggt
      481 cttgtagtta ccgtcatctt tgaaaaaaat agttctttct tgaacataac cttctggcat
      541 ggcagacttg aaaaagtcat gttgtttcat gtgatctggg tatctagaaa aacattgaac
      601 accataagtt aaagtagtga ctaaggttgg ccatggaact ggcaatttac cagtagtaca
      661 aataaatttt aaggtcaatt taccgtaagt agcatcacct tcaccttcac cggagacaga
      721 aaatttgtga ccattaacat caccatctaa ttcaaccaaa attgggacaa caccagtgaa
      781 taattcttca cctttagaca tagatctacc catcgacccc gtggagccgg ttgatcctgt
      841 agaaccagta ctagtctcgt cgactccgag ctctccgcat gccagcatat gattgaatta
      901 gttctaattt agtaattgaa
//
