LOCUS       sequence-76782-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of C-terminal yEGFP tagged gene 
            sequences together with the S. pombe spo5 gene DSR element into the 
            S. pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-cyEGFP-spo5DSR
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tacttcgtat ataataccat atacgaagtt atcccggggc tttgtctaac aggttttatg
       61 ttggtttaag tttgagtatg tgataagtgt ttgaaaagat ttcgatatta gagtttgatg
      121 ttcaagtaaa tgctggaaag atgtttaaat ttaattagca gcagaagtag cgggatgggc
      181 atgatatggc gtagtcccgg gagatcttta tttgtacaat tcatccatac catgggtaat
      241 accagcagca gtaacaaatt ctaacaagac catgtggtct ctcttttcgt ttggatcttt
      301 ggataaggca gattgagtgg ataagtaatg gttgtctggt aacaagactg gaccatcacc
      361 aattggagta ttttgttgat aatggtcagc taattgaaca gaaccatctt caatgttgtg
      421 tctaattttg aagttaactt tgataccatt cttttgtttg tcagccatga tgtaaacatt
      481 gtgagagtta tagttgtatt ccaatttgtg acctaaaatg ttaccatctt ctttaaaatc
      541 aatacctttt aattcgattc tattaactaa ggtatcacct tcaaacttga cttcagctct
      601 ggtcttgtag ttaccgtcat ctttgaaaaa aatagttctt tcttgaacat aaccttctgg
      661 catggcagac ttgaaaaagt catgttgttt catgtgatct gggtatctag aaaaacattg
      721 aacaccataa gttaaagtag tgactaaggt tggccatgga actggcaatt taccagtagt
      781 acaaataaat tttaaggtca atttaccgta agtagcatca ccttcacctt caccggagac
      841 agaaaatttg tgaccattaa catcaccatc taattcaacc aaaattggga c
//
