LOCUS       sequence-76784-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; used as 
            template for making expression cassette with multiple gRNA target 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pCBC-MT3T4
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        14..89
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        339..718
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     primer_bind     complement(750..769)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(751..769)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(776..793)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(776..792)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(785..807)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgccaaccaa cgagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
       61 aacttgaaaa agtggcaccg agtcggtgct tttttttgca aaattttcca gatcgatttc
      121 ttcttcctct gttcttcggc gttcaatttc tggggttttc tcttcgtttt ctgtaactga
      181 aacctaaaat ttgacctaaa aaaaatctca aataatatga ttcagtggtt ttgtactttt
      241 cagttagttg agttttgcag ttccgatgag ataaaccaat aagtaattca tccaggtcac
      301 caagttctag gattttcaga actgcaactt attttatcaa ggaatcttta aacatacgaa
      361 cagatcactt aaagttcttc tgaagcaact taaagttatc aggcatgcat ggatcttgga
      421 ggaatcagat gtgcagtcag ggaccatagc acaagacagg cgtcttctac tggtgctacc
      481 agcaaatgct ggaagccggg aacactgggt acgttggaaa ccacgtgatg tgaagaagta
      541 agataaactg taggagaaaa gcatttcgta gtgggccatg aagcctttca ggacatgtat
      601 tgcagtatgg gccggcccat tacgcaattg gacgacaaca aagactagta ttagtaccac
      661 ctcggctatc cacatagatc aaagctgatt taaaagagtt gtgcagatga tccgtggcgc
      721 ctttggccta tttggcctgg cccaattcgc cctatagtga gtcgtattac aattcactgg
      781 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      841 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      901 cccaacagtt gcgcagccta tacgtacggc agttt
//
