LOCUS       sequence-76787-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 
            promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUN6I11
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(322..342)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(349..414)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cctgtggaag aaagaatcgt caaccttcgc catctcgttg gagaaaatct cctgcaggta
       61 gcagatgcga ttcttcctgc gcgtgtaccg cctgcgggcg gtgcgcttga gccgcgtagc
      121 ctcagccgtc tcgccgctgt cgaacaggag agcgccaatg agattcttct tgatggaatg
      181 ccgatcggtg ttgcccagga ccttgaactt ctttgagggc accttgtact cgtcggtgat
      241 cacggcccag ccaacagagt tagtcccaat ggcgaggccg atcgagtact tcttgtcagc
      301 agctggcacc ccgtggatgc caaccttcct cttcttcttc ggagccatct tgtcatcatc
      361 atccttgtaa tcaatgtcgt ggtccttgta atccccgtcg tggtccttgt aatccatcta
      421 gagcggccgc ggccatacaa gtattgggga tcccccgggc tgcagaagta acaccaaaca
      481 acagggtgag catcgacaaa agaaacagta ccaagcaaat aaatagcgta tgaaggcagg
      541 gctaaaaaaa tccacatata gctgctgcat atgccatcat ccaagtatat caagatcaaa
      601 ataattataa aacatacttg tttattataa tagataggta ctcaaggtta gagcatatga
      661 atagatgctg catatgccat catgtatatg catcagtaaa acccacatca acatgtatac
      721 ctatcctaga tcgatatttc catccatctt aaactcgtaa ctatgaagat gtatgacaca
      781 cacatacagt tccaaaatta ataaatacac caggtagttt gaaacagtat tctactccga
      841 tctagaacga atgaacgacc gcccaaccac accacatcat cacaaccaag cgaacaaaaa
      901 gcatctctgt atatgcatca gtaaaacccg catcaacatg tatacctatc ctagatcgat
      961 atttccatcc atcatc
//
