LOCUS       sequence-76802-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; used as 
            template for making expression cassette with multiple gRNA target 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pCBC-DT2T3
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(496..571)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(638..657)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(639..657)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(664..681)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(664..680)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(673..695)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 attggtctct aaacgatcta gatttcccgg taacaatcac tacttcgtct ctaaccatat
       61 ataaactcag ctgctttctt tacctaagcg ctgtggtact tttgaagatt gttaatagct
      121 taaatgggcc tattttagaa aaaggcctat ccctcgcttc cttctcaagc ccatttatat
      181 tattgaattt tttcccaact aaagatttta ttttcttatg caagtctcac tcacactcac
      241 gtaaatttca aatataatcg tggtggaact aaaacaatga ccgtctcatc atatgtcctc
      301 attccctgtc atttcaaaga cctaattcaa atgtgattgt acaaaaatcc ccttcctata
      361 cacttttctt attccctaaa gctaaaacca ttattttcca tccaatgaat gtgcagaaaa
      421 tttactacac ccattggcca aggatcatga gagctgaaac acgcaaaaaa gctgggaaat
      481 tctatccaaa aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct
      541 tattttaact tgctatttct agctctaaaa ctgccattgt ataactttgc caatagagac
      601 cattattcct ttggcctatt tggcctggcc caattcgccc tatagtgagt cgtattacaa
      661 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      721 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      781 tcgcccttcc caacagttgc gcagcctata cgtacggcag tttaaggttt acacctataa
      841 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgccggg
      901 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tca
//
