LOCUS       sequence-76805-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat14
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    76..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             115..137
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             148..192
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             208..225
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat
       61 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
      121 taactttaag aaggagatat acatatgaaa gaaaccgctg ctgctaaatt cgaacgccag
      181 cacatggaca gcccagatct gggtaccctg gtgccacgcg gttccatggc gatatcggat
      241 cgggaaggtg tgaatgacaa tgaagaaggc tttttctctg ctcgtggtat taccgacatc
      301 ctggtggtgg acaatctgaa gttcctgttc gatgaatatg tgcgctattc atacgttgac
      361 gaaaacggcg aaaccaaatc gctggatgac tttctgatta aggtgcaggg tgaaatcccg
      421 gaaaacgcag atctgaaaac cgaagaactg ctgacgctgc cggctaatga agtcctgtgg
      481 cgcattctgg acgtgctgat cccgccggcg aagctgctga ttgaagaaga agcggccaaa
      541 gctcaggcgc tgtggacccg tctgtttgca gctgcgggcc aaaaaacgag tctgctggat
      601 tatattcgtg gtccggtggc aaccgttctg gtccgtctga cgggcctgga aggtgaacag
      661 ctgggcatcg ttagcctgcg cttcctggaa gaaggcgata aagttggtgc agcagtcggt
      721 gcaggtgccg gtaacgaaga acgttacccg gacctgcaga ttatcggcgg taatgtggct
      781 accgctgcag gtgcgcgtga aagtggtgca ctgtttgttg atcgcgctga aattgtcgcg
      841 tccttcgaac gtctgacctg gctgaacgtt aatga
//
