LOCUS       sequence-76807-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human connexin 43 with all internal methionines mutated to 
            leucine and a C_terminal HA epitope fusion tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Smyth JW, Shaw RM
  TITLE     Autoregulation of Connexin43 Gap Junction Formation by Internally 
            Translated Isoforms.
  JOURNAL   Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009.
  PUBMED    24210816
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009."
COMMENT     Sequence Label: pcDNA3.2-Cx43-ML-HA
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..68)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    85..109
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taaccggtac gcgtagactc gagtcattac taaccggtac gcgcgtaatc tgggacgtcg
       61 tatgggtacg ggccgcctag atcaaccact ttgtacaaga aagctgggta gatctccagg
      121 tcatcaggcc gaggtctgct gctggcacga ctgctggctc tgcttgaagg tcgctggtcc
      181 acaatggcta gtggctgtaa ttcatgtcca gcagctagtt tttttgaatt ctggttatca
      241 tcggggaaat caaaaggctg tgcatgggag ttagagatgg tgcttcccgc ctgccctagt
      301 cgattttgtt ctgcactgta attagcccag ttttgctcac ttgcttgctt gttgtaattg
      361 cggcaagaag aattgtttct gtcgccagta accagcttgt acccaggagg agatagaggc
      421 gagaggggag cggttggtga ggagcagcca ttgaaataag catatttttg agacccacag
      481 tctttggcag ggctcagcgc accactggtc gcatggtaag ggtcgctctt tcccttaacc
      541 cgatccttaa cgcccttgaa gaaaacatag aagagttcaa tgatattcaa ggccagggac
      601 accaaggaca ccaccagtaa gaagatgatg aagatggttt tctccgtggg gcgagagagg
      661 aaacagtcca cctgatgtgg gcagggatct cttttgcaag tgtaaacagc actcaagctg
      721 aatccataga tgtaccactg gatcagcaag aagg
//
