LOCUS       sequence-76809-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human connexin 43 with M100, M125, M147, and M213 mutated to
            L and wobble mutations conferring resistance against an siRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Smyth JW, Shaw RM
  TITLE     Autoregulation of Connexin43 Gap Junction Formation by Internally 
            Translated Isoforms.
  JOURNAL   Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009.
  PUBMED    24210816
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009."
COMMENT     Sequence Label: pcDNA3.2-Cx43-M100/125/147/213-siResist
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..28
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atcgaaatta atacgactca ctatagggag acccaagctg gctagttaag ctatcaacaa
       61 gtttgtacaa aaaagcaggc ttcaccatgg gtgactggag cgccttaggc aaactccttg
      121 acaaggttca agcctactca actgctggag ggaaggtgtg gctgtcagta cttttcattt
      181 tccgaatcct gctgctgggg acagcggttg agtcagcctg gggcgacgaa caatccgcat
      241 tccgctgtaa cactcagcaa cctggttgtg aaaatgtctg ctatgacaag tctttcccaa
      301 tctctcatgt gcgcttctgg gtcctgcaga tcatatttgt gtctgtaccc acactcttgt
      361 acctggctca tgtgttctat gtgctacgaa aggaagagaa actgaacaag aaagaggaag
      421 aactcaaggt tgcccaaact gatggtgtca atgtggacct acacttgaag cagattgaga
      481 taaagaagtt caagtacggt attgaagagc atggtaaggt gaaactacga ggggggttgc
      541 tgcgaaccta catcatcagt atcctcttca agtctatctt tgaggtggcc ttcttgctga
      601 tccagtggta catctatgga ttcagcttga gtgctgttta cacttgcaaa agagatccct
      661 gcccacatca ggtggactgt ttcctctctc gccccacgga gaaaaccatc ttcatcatct
      721 tcttactggt ggtgtccttg gtgtccctgg ccttgaatat cattgaactc ttctatgttt
      781 tcttcaaggg cgttaa
//
