LOCUS       sequence-76811-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes C-terminal fragment of human connexin 43 initiating at M213 
            with a V5 C-terminal fusion tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Smyth JW, Shaw RM
  TITLE     Autoregulation of Connexin43 Gap Junction Formation by Internally 
            Translated Isoforms.
  JOURNAL   Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009.
  PUBMED    24210816
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009."
COMMENT     Sequence Label: pDEST-mCherry-GJA1-20k-N1
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(581..605)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      619..628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagcatcaaa caagtttgta caaaaaagca
       61 ggcttcacca tgctggtggt gtccttggtg tccctggcct tgaatatcat tgaactcttc
      121 tatgttttct tcaagggcgt taaggatcgg gttaagggaa agagcgaccc ttaccatgcg
      181 accagtggtg cgctgagccc tgccaaagac tgtgggtctc aaaaatatgc ttatttcaat
      241 ggctgctcct caccaaccgc tcccctctcg cctctatctc ctcctgggta caagctggtt
      301 actggcgaca gaaacaattc ttcttgccgc aattacaaca agcaagcaag tgagcaaaac
      361 tgggctaatt acagtgcaga acaaaatcga ctagggcagg cgggaagcac catctctaac
      421 tcccatgcac agccttttga tttccccgat gataaccaga attcaaaaaa actagctgct
      481 ggacatgaat tacagccact agccattgtg gaccagcgac cttcaagcag agccagcagt
      541 cgtgccagca gcagacctcg gcctgatgac ctggagatct acccagcttt cttgtacaaa
      601 gtggttcgat gggatcccgc caccatggtg agcaagggcg aggaggataa catggccatc
      661 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      721 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc c
//
