LOCUS       sequence-76812-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes C-terminal fragment of human connexin 43 initiating at M213 
            with a V5 C-terminal fusion tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Smyth JW, Shaw RM
  TITLE     Autoregulation of Connexin43 Gap Junction Formation by Internally 
            Translated Isoforms.
  JOURNAL   Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009.
  PUBMED    24210816
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009."
COMMENT     Sequence Label: pDEST-mCherry-GJA1-20k-N1
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    136..160
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(677..701)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(735..834)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(764..784)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg
       61 aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct caccatggtg
      121 gcgggatccc atcgaaccac tttgtacaag aaagctgggt agatctccag gtcatcaggc
      181 cgaggtctgc tgctggcacg actgctggct ctgcttgaag gtcgctggtc cacaatggct
      241 agtggctgta attcatgtcc agcagctagt ttttttgaat tctggttatc atcggggaaa
      301 tcaaaaggct gtgcatggga gttagagatg gtgcttcccg cctgccctag tcgattttgt
      361 tctgcactgt aattagccca gttttgctca cttgcttgct tgttgtaatt gcggcaagaa
      421 gaattgtttc tgtcgccagt aaccagcttg tacccaggag gagatagagg cgagagggga
      481 gcggttggtg aggagcagcc attgaaataa gcatattttt gagacccaca gtctttggca
      541 gggctcagcg caccactggt cgcatggtaa gggtcgctct ttcccttaac ccgatcctta
      601 acgcccttga agaaaacata gaagagttca atgatattca aggccaggga caccaaggac
      661 accaccagca tggtgaagcc tgcttttttg tacaaacttg tttgatgcta gcggatctga
      721 cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt
      781 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
      841 aacaaactcc cattgacgtc aatggggt
//
