LOCUS       sequence-76835-e        5143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses proteorhodopsin using the Ppuf843-1200 promoter segment, 
            for use in R. sphaeroides .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Tikh IB, Held M, Schmidt-Dannert C
  TITLE     BioBrick compatible vector system for protein expression in 
            Rhodobacter sphaeroides.
  JOURNAL   Appl Microbiol Biotechnol. 2014 Feb 9.
  PUBMED    24509770
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol. 2014 Feb 9."
COMMENT     Sequence Label: pBBRBB-Ppuf843-1200-PR
FEATURES             Location/Qualifiers
     source          1..5143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(148..810)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      811..1580
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             3060..3854
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3114..3133)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3724..3743
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             5121..5138
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggccgcctcg agcatcacca tcaccatcac tgattctcct tacgcatctg tgcggtattt
       61 cacaccgcat gtactagtta acttcaggac ctgcagtcta gtatagtctg gaacagcgca
      121 cttacgggtt gctgcgcaac ccaagtgcta ccggcgcggc agcgtgaccc gtgtcggcgg
      181 ctccaacggc tcgccatcgt ccagaaaaca cggctcatcg ggcatcggca ggcgctgctg
      241 cccgcgccgt tcccattcct ccgtttcggt caaggctggc aggtctggtt ccatgcccgg
      301 aatgccgggc tggctgggcg gctcctcgcc ggggccggtc ggtagttgct gctcgcccgg
      361 atacagggtc gggatgcggc gcaggtcgcc atgccccaac agcgattcgt cctggtcgtc
      421 gtgatcaacc accacggcgg cactgaacac cgacaggcgc aactggtcgc ggggctggcc
      481 ccacgccacg cggtcattga ccacgtaggc cgacacggtg ccggggccgt tgagcttcac
      541 gacggagatc cagcgctcgg ccaccaagtc cttgactgcg tattggaccg tccgcaaaga
      601 acgtccgatg agcttggaaa gtgtcttctg gctgaccacc acggcgttct ggtggcccat
      661 ctgcgccacg aggtgatgca gcagcattgc cgccgtgggt ttcctcgcaa taagcccggc
      721 ccacgcctca tgcgctttgc gttccgtttg cacccagtga ccgggcttgt tcttggcttg
      781 aatgccgatt tctctggact gcgtggccat gcttatctcc atgcggtagg gtgccgcacg
      841 gttgcggcac catgcgcaat cagctgcaac ttttcggcag cgcgacaaca attatgcgtt
      901 gcgtaaaagt ggcagtcaat tacagatttt ctttaaccta cgcaatgagc tattgcgggg
      961 ggtgccgcaa tgagctgttg cgtacccccc ttttttaagt tgttgatttt taagtctttc
     1021 gcatttcgcc ctatatctag ttctttggtg cccaaagaag ggcacccctg cggggttccc
     1081 ccacgccttc ggcgcggctc cccctccggc aaaaagtggc ccctccgggg cttgttgatc
     1141 gactgcgcgg ccttcggcct tgcccaaggt ggcgctgccc ccttggaacc cccgcactcg
     1201 ccgccgtgag gctcgggggg caggcgggcg ggcttcgcct tcgactgccc ccactcgcat
     1261 aggcttgggt cgttccaggc gcgtcaaggc caagccgctg cgcggtcgct gcgcgagcct
     1321 tgacccgcct tccacttggt gtccaaccgg caagcgaagc gcgcaggccg caggccggag
     1381 gcttttcccc agagaaaatt aaaaaaattg atggggcaag gccgcaggcc gcgcagttgg
     1441 agccggtggg tatgtggtcg aaggctgggt agccggtggg caatccctgt ggtcaagctc
     1501 gtgggcaggc gcagcctgtc catcagcttg tccagcaggg ttgtccacgg gccgagcgaa
     1561 gcgagccagc cggtggccgc tcgcggccat cgtccacata tccacgggct ggcaagggag
     1621 cgcagcgacc gcgcagggcg aagcccggag agcaagcccg tagggcgccg cagccgccgt
     1681 aggcggtcac gactttgcga agcaaagtct agtgagtata ctcaagcatt gagtggcccg
     1741 ccggaggcac cgccttgcgc tgcccccgtc gagccggttg gacaccaaaa gggaggggca
     1801 ggcatggcgg catacgcgat catgcgatgc aagaagctgg cgaaaatggg caacgtggcg
     1861 gccagtctca agcacgccta ccgcgagcgc gagacgccca acgctgacgc cagcaggacg
     1921 ccagagaacg agcactgggc ggccagcagc accgatgaag cgatgggccg actgcgcgag
     1981 ttgctgccag agaagcggcg caaggacgct gtgttggcgg tcgagtacgt catgacggcc
     2041 agcccggaat ggtggaagtc ggccagccaa gaacagcagg cggcgttctt cgagaaggcg
     2101 cacaagtggc tggcggacaa gtacggggcg gatcgcatcg tgacggccag catccaccgt
     2161 gacgaaacca gcccgcacat gaccgcgttc gtggtgccgc tgacgcagga cggcaggctg
     2221 tcggccaagg agttcatcgg caacaaagcg cagatgaccc gcgaccagac cacgtttgcg
     2281 gctgctgtgg ccgatctagg gctgcaacgg ggcatcgagg gcagcaaggc acgtcacacg
     2341 cgcattcagg cgttctacga ggccctggag cggccaccag tgggccacgt caccatcagc
     2401 ccgcaagcgg tcgagccacg cgcctatgca ccgcagggat tggccgaaaa gctgggaatc
     2461 tcaaagcgcg ttgagacgcc ggaagccgtg gccgaccggc tgacaaaagc ggttcggcag
     2521 gggtatgagc ctgccctaca ggccgccgca ggagcgcgtg agatgcgcaa gaaggccgat
     2581 caagcccaag agacggcccg agaccttcgg gagcgcctga agcccgttct ggacgccctg
     2641 gggccgttga atcgggatat gcaggccaag gccgccgcga tcatcaaggc cgtgggcgaa
     2701 aagctgctga cggaacagcg ggaagtccag cgccagaaac aggcccagcg ccagcaggaa
     2761 cgcgggcgcg cacatttccc cgaaaagtgc cacctgggat gaatgtcagc tactgggcta
     2821 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     2881 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     2941 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     3001 ggatctgatg gcgcagggga tcaagatttg atcaagagac aggatgagga tcgtttcgca
     3061 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     3121 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     3181 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactac
     3241 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3301 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3361 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3421 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3481 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3541 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3601 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3661 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3721 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3781 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3841 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3901 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttggact agagaattca
     3961 tcctgaactt atctagattc ggcgagagga agggagagaa ccaatgagcg atcatgccgt
     4021 caacacgccg gtccatgccg ccagggccca cgggcaccga gcaccacgtg ccgagttcta
     4081 cgtctacttc gccgtcattc tgctgggcgc cttcccggtg gccttcgtga gctggatcgt
     4141 ctcgacgatc cgccaccgca ggcttcccaa gcgcggcccc ttcgcgtccg cctggttcga
     4201 tgccaaggcg atcacgccgc tgattttccg cgcctgaccg caggtcaggt tgcgacacgc
     4261 cattcgtcgt ctccccaagg ggcggcggat taatcgggag ggcatggtgc cttaccgtaa
     4321 cccacgccac cagcatgtgg cgtcagtctt acgatccgga ggatagcaga tctatgaaat
     4381 tattactgat attaggtagt gttattgcac ttcctacatt tgctgcaggt ggtggtgacc
     4441 ttgatgctag tgattacact ggtgtttctt tttggttagt tactgctgct ttattagcat
     4501 ctactgtatt tttctttgtt gaaagagata gagtttctgc aaaatggaaa acatcattaa
     4561 ctgtatctgg tcttgttact ggtattgctt tctggcatta catgtacatg agaggggtat
     4621 ggattgaaac tggtgattcg ccaactgtat ttagatacat tgattggtta ctaacagttc
     4681 ctctattaat atgtgaattc tacttaattc ttgctgctgc aactaatgtt gctggatcat
     4741 tatttaagaa attactagtt ggttctcttg ttatgcttgt gtttggttac atgggtgaag
     4801 caggaatcat ggctgcatgg cctgcattca ttattgggtg tttagcttgg gtatacatga
     4861 tttatgaatt atgggctgga gaaggaaaat ctgcatgtaa tactgcaagt cctgctgtgc
     4921 aatcagctta caacacaatg atgtatatta tcatctttgg ttgggcgatt tatcctgtag
     4981 gttatttcac aggttacctg atgggtgacg gtggatcagc tcttaactta aaccttatct
     5041 ataaccttgc tgactttgtt aacaagattc tatttggttt aattatatgg aatgttgctg
     5101 ttaaagaatc ttctaatgct catcaccatc atcaccatta agc
//
