LOCUS       sequence-76836-e        5869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressed DsRed using the Ppuf1-1200 promoter segment, for use in R.
            sphaeroides.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Tikh IB, Held M, Schmidt-Dannert C
  TITLE     BioBrick compatible vector system for protein expression in 
            Rhodobacter sphaeroides.
  JOURNAL   Appl Microbiol Biotechnol. 2014 Feb 9.
  PUBMED    24509770
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol. 2014 Feb 9."
COMMENT     Sequence Label: pBBRBB-Ppuf1-1200-DsRed
FEATURES             Location/Qualifiers
     source          1..5869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6..683
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(186..206)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     404..423
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     598..621
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             698..715
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(833..1495)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      1496..2265
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             3745..4539
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3799..3818)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4409..4428
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gatctatgga tagcactgag aacgtcatca agcccttcat gcgcttcaag gtgcacatgg
       61 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc aagccctacg
      121 agggcaccca gaccgccaag ctgcaggtga ccaagggcgg ccccctgccc ttcgcctggg
      181 acatcctgtc cccccagttc cagtacggct ccaaggtgta cgtgaagcac cccgccgaca
      241 tccccgacta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      301 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcaccttca
      361 tctaccacgt gaagttcatc ggcgtgaact tcccctccga cggccccgta atgcagaaga
      421 agactctggg ctgggagccc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
      481 gcgagatcca caaggcgctg aagctgaagg gcggcggcca ctacctggtg gagttcaagt
      541 caatctacat ggccaagaag cccgtgaagc tgcccggcta ctactacgtg gactccaagc
      601 tggacatcac ctcccacaac gaggactaca ccgtggtgga gcagtacgag cgcgccgagg
      661 cccgccacca cctgttccag taggcggccg cctcgagcat caccatcacc atcactgatt
      721 ctccttacgc atctgtgcgg tatttcacac cgcatgtact agttaacttc aggacctgca
      781 gtctagtata gtctggaaca gcgcacttac gggttgctgc gcaacccaag tgctaccggc
      841 gcggcagcgt gacccgtgtc ggcggctcca acggctcgcc atcgtccaga aaacacggct
      901 catcgggcat cggcaggcgc tgctgcccgc gccgttccca ttcctccgtt tcggtcaagg
      961 ctggcaggtc tggttccatg cccggaatgc cgggctggct gggcggctcc tcgccggggc
     1021 cggtcggtag ttgctgctcg cccggataca gggtcgggat gcggcgcagg tcgccatgcc
     1081 ccaacagcga ttcgtcctgg tcgtcgtgat caaccaccac ggcggcactg aacaccgaca
     1141 ggcgcaactg gtcgcggggc tggccccacg ccacgcggtc attgaccacg taggccgaca
     1201 cggtgccggg gccgttgagc ttcacgacgg agatccagcg ctcggccacc aagtccttga
     1261 ctgcgtattg gaccgtccgc aaagaacgtc cgatgagctt ggaaagtgtc ttctggctga
     1321 ccaccacggc gttctggtgg cccatctgcg ccacgaggtg atgcagcagc attgccgccg
     1381 tgggtttcct cgcaataagc ccggcccacg cctcatgcgc tttgcgttcc gtttgcaccc
     1441 agtgaccggg cttgttcttg gcttgaatgc cgatttctct ggactgcgtg gccatgctta
     1501 tctccatgcg gtagggtgcc gcacggttgc ggcaccatgc gcaatcagct gcaacttttc
     1561 ggcagcgcga caacaattat gcgttgcgta aaagtggcag tcaattacag attttcttta
     1621 acctacgcaa tgagctattg cggggggtgc cgcaatgagc tgttgcgtac cccccttttt
     1681 taagttgttg atttttaagt ctttcgcatt tcgccctata tctagttctt tggtgcccaa
     1741 agaagggcac ccctgcgggg ttcccccacg ccttcggcgc ggctccccct ccggcaaaaa
     1801 gtggcccctc cggggcttgt tgatcgactg cgcggccttc ggccttgccc aaggtggcgc
     1861 tgcccccttg gaacccccgc actcgccgcc gtgaggctcg gggggcaggc gggcgggctt
     1921 cgccttcgac tgcccccact cgcataggct tgggtcgttc caggcgcgtc aaggccaagc
     1981 cgctgcgcgg tcgctgcgcg agccttgacc cgccttccac ttggtgtcca accggcaagc
     2041 gaagcgcgca ggccgcaggc cggaggcttt tccccagaga aaattaaaaa aattgatggg
     2101 gcaaggccgc aggccgcgca gttggagccg gtgggtatgt ggtcgaaggc tgggtagccg
     2161 gtgggcaatc cctgtggtca agctcgtggg caggcgcagc ctgtccatca gcttgtccag
     2221 cagggttgtc cacgggccga gcgaagcgag ccagccggtg gccgctcgcg gccatcgtcc
     2281 acatatccac gggctggcaa gggagcgcag cgaccgcgca gggcgaagcc cggagagcaa
     2341 gcccgtaggg cgccgcagcc gccgtaggcg gtcacgactt tgcgaagcaa agtctagtga
     2401 gtatactcaa gcattgagtg gcccgccgga ggcaccgcct tgcgctgccc ccgtcgagcc
     2461 ggttggacac caaaagggag gggcaggcat ggcggcatac gcgatcatgc gatgcaagaa
     2521 gctggcgaaa atgggcaacg tggcggccag tctcaagcac gcctaccgcg agcgcgagac
     2581 gcccaacgct gacgccagca ggacgccaga gaacgagcac tgggcggcca gcagcaccga
     2641 tgaagcgatg ggccgactgc gcgagttgct gccagagaag cggcgcaagg acgctgtgtt
     2701 ggcggtcgag tacgtcatga cggccagccc ggaatggtgg aagtcggcca gccaagaaca
     2761 gcaggcggcg ttcttcgaga aggcgcacaa gtggctggcg gacaagtacg gggcggatcg
     2821 catcgtgacg gccagcatcc accgtgacga aaccagcccg cacatgaccg cgttcgtggt
     2881 gccgctgacg caggacggca ggctgtcggc caaggagttc atcggcaaca aagcgcagat
     2941 gacccgcgac cagaccacgt ttgcggctgc tgtggccgat ctagggctgc aacggggcat
     3001 cgagggcagc aaggcacgtc acacgcgcat tcaggcgttc tacgaggccc tggagcggcc
     3061 accagtgggc cacgtcacca tcagcccgca agcggtcgag ccacgcgcct atgcaccgca
     3121 gggattggcc gaaaagctgg gaatctcaaa gcgcgttgag acgccggaag ccgtggccga
     3181 ccggctgaca aaagcggttc ggcaggggta tgagcctgcc ctacaggccg ccgcaggagc
     3241 gcgtgagatg cgcaagaagg ccgatcaagc ccaagagacg gcccgagacc ttcgggagcg
     3301 cctgaagccc gttctggacg ccctggggcc gttgaatcgg gatatgcagg ccaaggccgc
     3361 cgcgatcatc aaggccgtgg gcgaaaagct gctgacggaa cagcgggaag tccagcgcca
     3421 gaaacaggcc cagcgccagc aggaacgcgg gcgcgcacat ttccccgaaa agtgccacct
     3481 gggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
     3541 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
     3601 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
     3661 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atttgatcaa
     3721 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     3781 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     3841 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     3901 ctgtccggtg ccctgaatga actacaggac gaggcagcgc ggctatcgtg gctggccacg
     3961 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4021 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     4081 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     4141 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     4201 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     4261 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     4321 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     4381 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     4441 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4501 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     4561 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     4621 atgaaaggtt ggactagaga attcatcctg aacttatcta gaatcgtcat gtaccgcgaa
     4681 ctcggccgcg ggctggccga ggtgctgggc aagccctacc tccaggcccc catcggggtc
     4741 gagagcacga ccgccttcct gcgccgcctg ggcgagattc tgggcctcga tccggagccc
     4801 ttcatcgagc gcgagaagca ctcgacgctg aagcccgtgt gggatctgtg gcggagtgtc
     4861 acgcaggact tcttcgggac ggccaatttc ggaatcgtgg cgaccgaaac ttatgcaaga
     4921 ggcatccgaa actatctcga aggcgatctc gggctgccct gcgccttcgc cgtggcccgc
     4981 aagaggggct cgaagaccga caacgaagcg gtgcgcggac tgatccgcca gcaccgtccg
     5041 ctcgtgctca tggggtcgat caacgagaag atttaccttg cggaactgaa agccggtcac
     5101 ggcccgcaac cctctttcat cgctgcctct ttcccgggtg cggcgatccg gcgcgctacc
     5161 ggaacgcccg ttatgggata tgcaggtgct acgtggttac tacaggaagt ttgcaacgcc
     5221 ctgttcgacg ccctgttcca cattctgccc ctcgggacgg agatggacag cgccgccgcc
     5281 acaccgacga cactgcgccg cgacttcccg tgggatgccg atgcgcaagc ggccctggac
     5341 cgcatcgtag aggagcatcc ggttctcacc cggatcagcg ccgcgcgtgc cttgcgcgac
     5401 gccgccgaga aggctgccct cgatgccggt gccgagaggg tcgtgagaga gactgtcgaa
     5461 gccctgcgtg ggccgggctt cggcgagagg aagggagaga accaatgagc gatcatgccg
     5521 tcaacacgcc ggtccatgcc gccagggccc acgggcaccg agcaccacgt gccgagttct
     5581 acgtctactt cgccgtcatt ctgctgggcg ccttcccggt ggccttcgtg agctggatcg
     5641 tctcgacgat ccgccaccgc aggcttccca agcgcggccc cttcgcgtcc gcctggttcg
     5701 atgccaaggc gatcacgccg ctgattttcc gcgcctgacc gcaggtcagg ttgcgacacg
     5761 ccattcgtcg tctccccaag gggcggcgga ttaatcggga gggcatggtg ccttaccgta
     5821 acccacgcca ccagcatgtg gcgtcagtct tacgatccgg aggatagca
//
