LOCUS       sequence-76840-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; used as 
            template for making expression cassette with multiple gRNA target 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pCBC-DT3T4
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        18..93
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(750..769)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(751..769)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(776..793)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(776..792)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(785..807)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 accgggaaat ctagatcgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
       61 tatcaacttg aaaaagtggc accgagtcgg tgcttttttt tggcaaaaat tttcagattt
      121 tttcttcatc tgtagatttc tgggtttttt tttccgtttc gtgaatcata agtgaagttt
      181 tggatgcaaa tctgcgcgaa aaaagttgga cctgcaatga gcttatttag atagctaaga
      241 caaagtgatt ggtccgttcg acttgccttc cgcacaatac atcatttctt cttagctttt
      301 tttcttcttc ttcgttcata cagttttttt ttgtttatca gcttacattt tcttgaaccg
      361 tagctttcgt tttcttcttt ttaactttcc attcggagtt tttgtatctt gtttcatagt
      421 ttgtcccagg attagaatga ttaggcatcg aaccttcaag aatttgattg aataaaacat
      481 cttcattctt aagatatgaa gataatcttc aaaaggcccc tgggaatctg aaagaagaga
      541 agcaggccca tttatatggg aaagaacaat agtatttctt atataggccc atttaagttg
      601 aaaacaatct tcaaaagtcc cacatcgctt agataagaaa acgaagctga gtttatatac
      661 agctagagtc gaagtagtga ttgagaagag atatggaaga tggtttagag accaataatc
      721 ctttggccta tttggcctgg cccaattcgc cctatagtga gtcgtattac aattcactgg
      781 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      841 cagcacatcc ccctttcgcc agctggcgta ata
//
