LOCUS       sequence-76845-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; used as 
            template for making expression cassette with multiple gRNA target 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pCBC-MT1T2
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        14..89
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tgccaaccaa cgagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
       61 aacttgaaaa agtggcaccg agtcggtgct tttttttttc gttttgcatt gagttttctc
      121 cgtcgcatgt ttgcagtttt attttccgtt ttgcattgaa atttctccgt ctcatgtttg
      181 cagcgtgttc aaaaagtacg cagctgtatt tcacttattt acggcgccac attttcatgc
      241 cgtttgtgcc aactatcccg agctagtgaa tacagcttgg cttcacacaa cactggtgac
      301 ccgctgacct gctcgtacct cgtaccgtcg tacggcacag catttggaat taaagggtgt
      361 gatcgatact gcttgctgct catgaatcca aaccacacgg agttcaaatt cccacagatt
      421 aaggctcgtc cgtcgcacaa ggtaatgtgt gaatattata tctgtcgtgc aaaattgcct
      481 ggcctgcaca attgctgtta tagttggcgg cagggagagt tttaacattg actagcgtgc
      541 tgataatttg tgagaaataa taattgacaa gtagatactg acatttgaga agagcttctg
      601 aactgttatt agtaacaaaa atggaaagct gatgcacgga aaaaggaaag aaaaagccat
      661 actttttttt aggtaggaaa agaaaaagcc atacgagact gatgtctctc agatgggccg
      721 ggatctgtct atctagcagg cagcagccca ccaacctcac gggccagcaa ttacgagtcc
      781 ttctaaaagc tcccgccgag gggcgctggc gctgctgtgc agcagcacgt ctaacattag
      841 tcccacctcg ccagtttaca gggagcagaa ccagcttata agcggaggcg cggcaccaag
      901 aagcgtgacc aggaggagca tgcccctttg gcctatttgg cctgg
//
