LOCUS       sequence-76848-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (OsU3 promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUN411
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(323..343)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(350..415)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gcctgtggaa gaaagaatcg tcaaccttcg ccatctcgtt ggagaaaatc tcctgcaggt
       61 agcagatgcg attcttcctg cgcgtgtacc gcctgcgggc ggtgcgcttg agccgcgtag
      121 cctcagccgt ctcgccgctg tcgaacagga gagcgccaat gagattcttc ttgatggaat
      181 gccgatcggt gttgcccagg accttgaact tctttgaggg caccttgtac tcgtcggtga
      241 tcacggccca gccaacagag ttagtcccaa tatcgaggcc gatcgagtac ttcttgtcag
      301 cagctggcac cccgtggatg ccaaccttcc tcttcttctt cggagccatc ttgtcatcat
      361 catccttgta atcaatgtcg tggtccttgt aatccccgtc gtggtccttg taatccatct
      421 agagcggccg cggccataca agtattgggg atcccccggg ctgcagaagt aacaccaaac
      481 aacagggtga gcatcgacaa aagaaacagt accaagcaaa taaatagcgt atgaaggcag
      541 ggctaaaaaa atccacatat agctgctgca tatgccatca tccaagtata tcaagatcaa
      601 aataattata aaacatactt gtttattata atagataggt actcaaggtt agagcatatg
      661 aatagatgct gcatatgcca tcatgtatat gcatcagtaa aacccacatc aacatgtata
      721 cctatcctag atcgatattt ccatccatct taaactcgta actatgaaga tgtatgacac
      781 acacatacag ttccaaaatt aataaataca ccaggtagtt tgaaacagta ttctactccg
      841 atctagaacg aatgaacgac cgcccaacca caccacatca tcacaaccaa gcgaacaaaa
      901 agcatctctg tatatgcatc agtaaaaccc gcatcaacat gtatacctat cctagatcga
      961 tatttccatc catcatcttc a
//
