LOCUS       sequence-76852-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 
            promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHSN6I01
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(311..331)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(338..403)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(587..633)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(622..642)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 aagaatcgtc aaccttcgcc atctcgttgg agaaaatctc ctgcaggtag cagatgcgat
       61 tcttcctgcg cgtgtaccgc ctgcgggcgg tgcgcttgag ccgcgtagcc tcagccgtct
      121 cgccgctgtc gaacaggaga gcgccaatga gattcttctt gatggaatgc cgatcggtgt
      181 tgcccaggac cttgaacttc tttgagggca ccttgtactc gtcggtgatc acggcccagc
      241 caacagagtt agtcccaatg gcgaggccga tcgagtactt cttgtcagca gctggcaccc
      301 cgtggatgcc aaccttcctc ttcttcttcg gagccatctt gtcatcatca tccttgtaat
      361 caatgtcgtg gtccttgtaa tccccgtcgt ggtccttgta atccatctag agcggccgcg
      421 gccatacaag tattggggat ccccctcgag tatcgttcgt aaatggtgaa aattttcaga
      481 aaattgcttt tgctttaaaa gaaatgattt aaattgctgc aatagaagta gaatgcttga
      541 ttgcttgaga ttcgtttgtt ttgtatatgt tgtgttgagg tcgaggtcct ctccaaatga
      601 aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc
      661 cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct
      721 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
      781 gcatcttcaa cgatggcctt tcctttatcg caatgatggc atttgtagga gccaccttcc
      841 ttttccacta tcttcacaat aaagtgacag atagctgggc aatggaatcc ga
//
