LOCUS       sequence-76856-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  TITLE     Johnson lab plasmids transferred from original pMEX to pcDNA
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA 3.1(-) mouse C/EBP beta (LIP)
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      450..459
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(542..561)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(543..561)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(606..809)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(635..655)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cgagctcgga tccgtcgacc tagcagtggc ccgccgaggc cagcagcggc tcgggcagct
       61 gcttgaacaa gttccgcagg gtgctgagct ctcgcgacag ctgctccacc ttcttctgca
      121 gccgctcgtt ctccgccgtc agctccagca ccttgtgctg cgtctccagg ttgcgcatct
      181 tggccttgtc gcggctcttg cgcaccgcga tgttgttgcg ctcgcgccgc atcttgtact
      241 cgtcgctcag cttgtccacc gtcttcttgg ccttggcctt ggcgggcgcg gccggcggcc
      301 ccgcgaagca ggcggcgggc gcggccttgg cgtcggcggg gctcggcgtg ccgggcgggc
      361 tggacgacga cgacgtggac aggctgccgc tgctgccgct cggcgtcgcc tggtagccca
      421 ggtaggcgcg cagggcgaac gggaaaccgg ccgccatggt gcgggaattc tgcagatatc
      481 cagcacagtg gcggccgctc gagtctagag ggcccgttta aacgctagcc agcttgggtc
      541 tccctatagt gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc
      601 cagagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat
      661 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
      721 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc
      781 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg
      841 tactg
//
