LOCUS       sequence-76867-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:mTurquoise2-Tub1+3'UTR::LEU2
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(27..737,738..740,741..743))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(69..90)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     418..437
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     677..698
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atactaataa cttctctcat ggatcccttg tacagctcgt ccatgccgag agtgatcccg
       61 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      121 agcttggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      181 ggggtgttct gctggtagtg gtcggcgagc tgcacgccgc cgtcctcgat gttgtggcgg
      241 atcttgaagt tggccttgat gccgttcttc tgcttgtcgg cggtgatata gacgttgtcg
      301 ctaaagtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      361 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      421 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
      481 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa gcactgcacg
      541 ccccaggaca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
      601 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
      661 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
      721 agctcctcgc ccttgctcac catgctttgc cttcgtttat cttgcctgct cattttttag
      781 tatattcttc gaagaaatca cattacttta tataatgtat aattcattat gtgataatgc
      841 caatcgctaa gaaaaaaaaa gagtcatcc
//
