LOCUS       sequence-76868-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:CloverGFP-Tub1+3'UTR::LEU2
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     277..298
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(350..536)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
ORIGIN
        1 acttcacctc ggcgcgggtc ttgtaggtac cgtcgtcctt gaaagagatg gtgcgctcct
       61 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
      121 agcggctgaa gcaggccacg ccgtagccga aggtggtcac gagggtgggc cagggcacgg
      181 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgttggtg gcatcgccct
      241 cgccctcgcc gcggacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
      301 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catctcgagc tttgccttcg
      361 tttatcttgc ctgctcattt tttagtatat tcttcgaaga aatcacatta ctttatataa
      421 tgtataattc attatgtgat aatgccaatc gctaagaaaa aaaaagagtc atccgctagg
      481 tggaaaaaaa aaatgaaaat cattaccgag gcataaaaaa atatagagtg tactagagga
      541 ggccaagagt aatagggtac cagcttttgt tccctttagt ggttaattaa ggcgcgcccc
      601 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tcgaccggtc
      661 gaggagaact tctagtatat ctacatacct aatattattg ccttattaaa aatggaatcc
      721 caacaattac atcaaaatcc acattctctt caaaatcaat tgtcctgtac ttccttgttc
      781 atgtgtgttc aaaaacgtta tatttatagg ataattatac tctatttctc aacaagtaat
      841 tggttgtttg gccgagcggt ctaaggcgcc tgattcaaga aatatcttga ccgcagttaa
      901 ctgtgggaat actcaggtat cgtaagatgc aagagttcga atctctta
//
