LOCUS       sequence-76872-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:Venus-Tub1+3'UTR::LEU2
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..741)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 aatactaata acttctctca tggatccttt gtacaattca tccataccat gggtaatacc
       61 agcagcagta acaaattcta acaagaccat gtggtctctc ttttcgtttg gatctttgga
      121 taaggcagat tgataggata agtaatggtt gtctggtaac aagactggac catcaccaat
      181 tggagtattt tgttgataat ggtcagctaa ttgaacacca ccatcttcaa tgttgtgtct
      241 aattttgaag ttagctttga taccattctt ttgtttgtca gcagtgatgt aaacattgtg
      301 agagttatag ttgtattcca atttgtgacc taaaatgtta ccatcttctt taaaatcaat
      361 accttttaat tcgattctat taactaaggt atcaccttca aacttgactt cagctctggt
      421 cttgtagtta ccgtcatctt tgaaaaaaat agttctttct tgaacataac cttctggcat
      481 ggcagacttg aaaaagtcat gttgtttcat atgatctggg tatctagcaa aacattgcaa
      541 accataacct aaagtagtga ctaaggttgg ccatggaact ggcaatttac cagtagtaca
      601 aatcaatttt aaggtcaatt taccgtaagt agcatcacct tcaccttcac cggagacaga
      661 aaatttgtga ccattaacat caccatctaa ttcaaccaaa attgggacaa caccagtgaa
      721 taattcttca cctttagaca tgctttgcct tcgtttatct tgcctgctca ttttttagta
      781 tattcttcga agaaatcaca ttactttata taatgtataa ttcattatgt gataatgcca
      841 atcgctaaga aaaaaaaaga gtcatccgct
//
