LOCUS       sequence-76874-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:mRuby2-Tub1+3'UTR::LEU2
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(58..768)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 agcctggacg ggcaaatcga acataccatt aatactaata acttctctca tggatccctt
       61 gtacagctcg tccatcccac caccaagccc ggcgaacttg gcaactgcgt gttcgcgttg
      121 tactacgaac atttcattgt cactttcctc taacctttcc aggcggtgat caacggcatg
      181 gataccgggc atcttgatgt tcccgacggt cttttttgac ctgtaagttg ttacgaaaga
      241 gcaagacaga tggccaccac catcaacttt cagtgccata tgagtgtatc ccctcagacc
      301 accatctgct ggatacatca tctctgtatt aggctcccaa cccttggtct tcttctgcat
      361 cacgggacca ttggagggaa agtttacccc tctgacttgg acgtggtaaa cgagacagcc
      421 atcctcaagg ctggtgtcct gcatgacggt gacgactcca ccatcttcgt atctcgtaac
      481 tctttcccaa gtaaaaccct caggaaagga ctgtttaaag aaatcaggaa tgcctttcgg
      541 gtacttgata aaagtacggc tgccatacat gaacgacgtg gcaagaatgt caaaggcaaa
      601 tggcaggggt cctccctcga tgactttgat cctcatggtt tgagttccca tgtacggatt
      661 gccttctcct tcacctgtgc atttgaattg gtggccgttg accgaacctt ccatgaccac
      721 cttcatacgc atattttcct tgatcagctc ttcgccctta gacaccatgc tttgccttcg
      781 tttatcttgc ctgctcattt tttagtatat tcttcgaaga aatcacatta ctttatataa
      841 tgtataattc attatgtgat aatgccaatc gctaagaaaa aaaaagagtc atccgctagg
      901 tggaaaaaaa aaatgaaaat cattaccgag gcataaaaa
//
