LOCUS       sequence-76879-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:CloverGFP-Tub1+3'UTR::TRP1
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(18..39)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     626..647
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtaatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
       61 tctttgctca gggcggactg atggctcagg tagtggttgt cgggcagcag cacggggccg
      121 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcaacg
      181 ttgtggcgga tcttgaagtt agccttgatg ccgttcttct gcttgtcggc cgtgatatag
      241 acgttgtggc tgttgaagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      301 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      361 gcgcgggtct tgtaggtacc gtcgtccttg aaagagatgg tgcgctcctg gacgtagcct
      421 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag
      481 caggccacgc cgtagccgaa ggtggtcacg agggtgggcc agggcacggg cagcttgccg
      541 gtggtgcaga tgaacttcag ggtcagcttg ccgttggtgg catcgccctc gccctcgccg
      601 cggacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
      661 ccggtgaaca gctcctcgcc cttgctcacc atctcgagct ttgccttcgt ttatcttgcc
      721 tgctcatttt ttagtatatt cttcgaagaa atcacattac tttatataat gtataattca
      781 ttatgtgata atgccaatcg ctaagaaaaa aaaagagtca tccgctaggt ggaaaaaaaa
      841 aatgaaaatc attaccga
//
