LOCUS       sequence-76885-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Shen WF, Chang CP, Rozenfeld S, Sauvageau G, Humphries RK, Lu M, 
            Lawrence HJ, Cleary ML, Largman C
  TITLE     Hox homeodomain proteins exhibit selective complex stabilities with 
            Pbx and DNA.
  JOURNAL   Nucleic Acids Res 1996 Mar 1;24(5):898-906.
  PUBMED    8600458
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "1996-03-1"; volume: "24(5)"; pages: "898-906"
COMMENT     Sequence Label: HOXC4 (human) HIS-tag pET
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    70..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             150..167
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             177..194
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             198..230
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
      121 taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc agcggcctgg
      181 tgccgcgcgg cagccatatg gctagcatga ctggtggaca gcaaatgggt cgggatccga
      241 attcgagctc ggatccacca tggtcatgag ctcggatcca ccatgctcat gagctcgtat
      301 ttgatggact ctaactacat cgatccgaaa tttcctccat gcgaagaata ttcgcaaaat
      361 agctacatcc ctgaacacag tccggaatat tacggccgga ccagggaatc gggattccag
      421 catcaccacc aggagctgta cccaccaccg cctccgcgcc ctagctaccc tgagcgccag
      481 tatagctgca ccagtctcca ggggcccggc aattcgcgag gccacgggcc ggcccaggcg
      541 ggccaccacc accccgagaa atcacagtcg ctctgcgagc cggcgcctct ctcaggcgcc
      601 tccgcctccc cgtccccagc cccgccagcc tgcagccagc cagcccccga ccatccctcc
      661 agcgccgcca gcaagcaacc catagtctac ccatggatga aaaaaattca cgttagcacg
      721 gtgaacccca attataacgg aggggaaccc aagcgctcga gggcagccta tacccggcag
      781 caagtcctgg aattagagaa agagtttcat tacaaccgct acctga
//
