LOCUS       sequence-76886-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Kraynov VS, Chamberlain C, Bokoch GM, Schwartz MA, Slabaugh S, Hahn 
            KM
  TITLE     Localized Rac activation dynamics visualized in living cells.
  JOURNAL   Science. 2000 Oct 13. 290(5490):333-7.
  PUBMED    11030651
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2000 Oct 13. 290(5490):333-7."
COMMENT     Sequence Label: pcDNA3-PBD-EYFP
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(397..418)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgc caacatgaat aagaagaaag agaaagagcg gccagagatt
      121 tctctccctt cagattttga acacacaatt catgtcggtt ttgatgctgt cacaggggag
      181 tttacgggaa tgccagagca gtgggcccgc ttgcttcaga catcaaatat cactaagtcg
      241 gagcagaaga aaaacccgca ggctgttctg gatgtgttgg agttttacaa ctcgaagaag
      301 acatccaaca gccagaaata catgagcttt acagataagt cacctagcgg ccgcgtgagc
      361 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      421 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      481 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      541 accttcggct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
      601 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      661 gacggcnact acnagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      721 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
      781 tacaac
//
