LOCUS       sequence-76889-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Kraynov VS, Chamberlain C, Bokoch GM, Schwartz MA, Slabaugh S, Hahn 
            KM
  TITLE     Localized Rac activation dynamics visualized in living cells.
  JOURNAL   Science. 2000 Oct 13. 290(5490):333-7.
  PUBMED    11030651
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2000 Oct 13. 290(5490):333-7."
COMMENT     Sequence Label: pcDNA3-PBD-EYFP
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(290..309)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(291..309)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(354..557)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(383..403)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        558..936
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgcggccgct aggtgactta tctgtaaagc tcatgtattt ctggctgttg gatgtcttct
       61 tcgagttgta aaactccaac acatccagaa cagcctgcgg gtttttcttc tgctccgact
      121 tagtgatatt tgatgtctga agcaagcggg cccactgctc tggcattccc gtaaactccc
      181 ctgtgacagc atcaaaaccg acatgaattg tgtgttcaaa atctgaaggg agagaaatct
      241 ctggccgctc tttctctttc ttcttattca tgttggcaag cttgggtctc cctatagtga
      301 gtcgtattaa tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg
      361 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
      421 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
      481 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
      541 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
      601 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
      661 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
      721 taccgtnnta ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
      781 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
      841 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
      901 ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg gcccgtacat
      961 cgcga
//
