LOCUS       sequence-76907-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:CloverGFP-Tub1+3'UTR::HIS3
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(1..708,709..711,712..714))
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELY"
     primer_bind     complement(40..61)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     648..669
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(721..907)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
ORIGIN
        1 gtacagctcg tccatgccat gtgtaatccc ggcggcggtc acgaactcca gcaggaccat
       61 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgatggctca ggtagtggtt
      121 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
      181 ctgcacgctg ccgtcctcaa cgttgtggcg gatcttgaag ttagccttga tgccgttctt
      241 ctgcttgtcg gccgtgatat agacgttgtg gctgttgaag ttgtactcca gcttgtgccc
      301 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
      361 gtcgccctcg aacttcacct cggcgcgggt cttgtaggta ccgtcgtcct tgaaagagat
      421 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
      481 gtggtcgggg tagcggctga agcaggccac gccgtagccg aaggtggtca cgagggtggg
      541 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgttggt
      601 ggcatcgccc tcgccctcgc cgcggacgct gaacttgtgg ccgtttacgt cgccgtccag
      661 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatctcgag
      721 ctttgccttc gtttatcttg cctgctcatt ttttagtata ttcttcgaag aaatcacatt
      781 actttatata atgtataatt cattatgtga taatgccaat cgctaagaaa aaaaaagagt
      841 catccgctag gtggaaaaaa aaaatgaaaa tcattaccga ggcataaaaa aatatagagt
      901 gtactagag
//
