LOCUS       sequence-76933-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG)
            upstream open reading from element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Calvo SE, Pagliarini DJ, Mootha VK
  TITLE     Upstream open reading frames cause widespread reduction of protein 
            expression and are polymorphic among humans.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 May 5;106(18):7507-12. doi: 
            10.1073/pnas.0810916106. Epub 2009 Apr 16.
  PUBMED    19372376
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0810916106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-05-5- 5"; volume: "106"; issue: "18"; pages: "7507-12"
COMMENT     Sequence Label: psicheck-2-Mut mod
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agagtactta atacgactca ctataggcta gcggcgtctg ggttcggttc tgaagtccgg
       61 gcttggtccg ggtgatcccc aaacactgac cgttctcatt tccttttggg agtattcttg
      121 cttcaccatg ttgagagcta agctagccac cttggcttcc aaggtgtacg accccgagca
      181 acgcaaacgc atgatcactg ggcctcagtg gtgggctcgc tgcaagcaaa tgaacgtgct
      241 ggactccttc atcaactact atgattccga gaagcacgcc gagaacgccg tgatttttct
      301 gcatggtaac gctgcctcca gctacctgtg gaggcacgtc gtgcctcaca tcgagcccgt
      361 ggctagatgc atcatccctg atctgatcgg aatgggtaag tccggcaaga gcgggaatgg
      421 ctcatatcgc ctcctggatc actacaagta cctcaccgct tggttcgagc tgctgaacct
      481 tccaaagaaa atcatctttg tgggccacga ctggggggct tgtctggcct ttcactactc
      541 ctacgagcac caagacaaga tcaaggccat cgtccatgct gagagtgtcg tggacgtgat
      601 cgagtcctgg gacgagtggc ctgacatcga ggaggatatc gccctgatca agagcgaaga
      661 gggcgagaaa atggtgcttg agaataactt cttcgtcgag accatgctcc caagcaagat
      721 catgcggaaa ctggagcctg aggagttcgc tgcctacctg gagccattca agga
//
