LOCUS       sequence-7694-ex        5170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Deuschle K, Chaudhuri B, Okumoto S, Lager I, Lalonde S, Frommer WB
  TITLE     Rapid Metabolism of Glucose Detected with FRET Glucose Nanosensors 
            in Epidermal Cells and Intact Roots of Arabidopsis RNA-Silencing 
            Mutants.
  JOURNAL   Plant Cell. 2006 Sep . 18(9):2314-25.
  PUBMED    16935985
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2006 Sep . 18(9):2314-25."
COMMENT     Sequence Label: pRSET FLIPglu-600uDelta11 Ares-Aphrodite
FEATURES             Location/Qualifiers
     source          1..5170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..38
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             67..89
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             109..126
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             130..162
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(166..174,175..189)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(221..242)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(482..501)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     829..850
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2516..2534)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2530..2577
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2674..3129
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2761..2780)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2971..2992
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3210..3314
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3315..3383,3384..4175)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLID*MEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4346..4934
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4835..4854
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5088..5105
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atctcgatcc cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta
       61 gataattttg tttaacttta agaaggagat atacatatgc ggggttctca tcatcatcat
      121 catcatggta tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat
      181 gacgataagg atccagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      241 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      301 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      361 gaccaccctg acctggggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
      421 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      481 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      541 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      601 ggagtacaac tacatcagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat
      661 caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
      721 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      781 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      841 ggagttcgtg accgccgccg ggatggtacc gctgatactc gcattggtgt aacaatctat
      901 aagtacgacg ataacgcgat gtctgtagtg cgcaaggcta ttgagcaaga tgcgaaagcc
      961 gcgccagatg ttcagctgct gatgaatgat tctcagaatg accagtccaa gcagaacgat
     1021 cagatcgacg tattgctggc gaaaggggtg aaggcactgg caatcaacct ggttgacccg
     1081 gcagctgcgg gtacggtgat tgagaaagcg cgtgggcaaa acgtgccggt ggttttcttc
     1141 aacaaagaac cgtctcgtaa ggcgctggat agctacgaca aagcctacta cgttggcact
     1201 gactccaaag agtccggcat tattcaaggc gatttgattg ctaaacactg ggcggcgaat
     1261 cagggttggg atctgaacaa agacggtcag attcagttcg tactgctgaa aggtgaaccg
     1321 ggccatccgg atgcagaagc acgtaccact tacgtgatta aagaattgaa cgataaaggc
     1381 atcaaaactg aacagttaca gttagatacc gcaatgtggg acaccgctca ggcgaaagat
     1441 aagatggacg cctggctgtc tggcccgaac gccaacaaaa tcgaagtggt tatcgccaac
     1501 aacgatgcga tggcaatggg cgcggttgaa gcgctgaaag cacacaacaa gtccagcatt
     1561 ccggtgtttg gcgtcgatgc gctgccagaa gcgctggcgc tggtgaaatc cggtgcactg
     1621 gcgggcaccg tactgaacga tgctaacaac caggcgaaag cgacctttga tctggcgaaa
     1681 aacctggccg atggtaaagg tgcggctgat ggcaccaact ggaaaatcga caacaaagtg
     1741 gtccgcgtac cttatgttgg cgtagataaa gacaacctgg ctctcgagtt gtttacgggc
     1801 gtcgtcccga tcctcgtgga actcgacggg gatgttaacg ggcataagtt ttcggtcagc
     1861 ggggaagggg agggggacgc gacgtatggg aagctcactc tcaagctgat ctgtacgacg
     1921 gggaaactcc cggtcccgtg gccgacgctg gtcacgacgc tgggatacgg gctccaatgc
     1981 tttgcgaggt atccggacca catgaaacag catgactttt tcaaatcggc gatgccggag
     2041 ggatacgtgc aggaacggac gatctttttc aaagacgatg ggaactataa gacgcgggcg
     2101 gaagtcaagt ttgaagggga cacgctcgtc aaccggatcg aactcaaggg gattgacttc
     2161 aaagaggatg ggaacatact cggccataag ctcgaataca attacaactc gcataacgta
     2221 tacatcaccg cggataagca aaagaatggg atcaaagcca atttcaaaat ccggcataac
     2281 atagaggatg ggggggtcca actggcggat cactatcagc aaaacacgcc gataggggat
     2341 gggccggtcc tcctcccgga taaccattac ctctcgtacc aaagcgcgct ctcgaaggac
     2401 ccgaatgaga aacgggacca catggttctc ctggagttcg tcacggcggc gggcatatag
     2461 aagcttgatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     2521 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     2581 aaaggaggaa ctatatccgg atctggcgta atagcgaaga ggcccgcacc gatcgccctt
     2641 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     2701 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     2761 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     2821 taaatcgggg gctcccttta gggttccgat ttagagcttt acggcacctc gaccgcaaaa
     2881 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     2941 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3001 tcaaccctat cgcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3061 ggttaaaaaa tgagctgatt taacaaatat ttaacgcgaa ttttaacaaa atattaacgt
     3121 ttacaatttc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     3181 atacaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3241 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3301 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3361 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     3421 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3481 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     3541 atacactatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     3601 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3661 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3721 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3781 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgaga
     3841 gtgacaccac gatgcctgta gcaatgccaa caacgttgcg caaactatta actggcgaac
     3901 tacttactct agcttcccgg caacaattaa tagactgaat ggaggcggat aaagttgcag
     3961 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4021 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag cgctcccgta
     4081 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4141 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4201 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4261 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4321 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4381 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4441 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     4501 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4561 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4621 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4681 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4741 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4801 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4861 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4921 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctgggc ttttgctggc
     4981 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     5041 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     5101 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     5161 attaatgcag
//
