LOCUS       sequence-76940-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human eIF2C1 mutant in mamalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells 
            requires Dicer and eIF2C translation initiation factors.
  JOURNAL   Curr Biol. 2003 Jan 8;13(1):41-6.
  PUBMED    12526743
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2003-01-8"; volume: "13(1)"; pages: "41-6"
COMMENT     Sequence Label: pcDNAM-human eIF2C1-A-Myc
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagttaa gcttggtacc gagctcggat ccgggccgcc
      121 tgacctccgc acgggtatat gggatggaag cgggaccctc gggagcagct gcgggcgctt
      181 acctgccccc cctgcagcag gtgttccagg cacctcgccg gcctggcatt ggcactgtgg
      241 ggaaaccaat caagctcctg gccaattact ttgaggtggg catccctaag atcgacgtgt
      301 accactacga ggtggacatc aagccggata agtgtccccg tagagtcaac cgggaagtgg
      361 tggaatacat ggtccagcat ttcaagcctc agatctttgg tgatcgcaag cctgtgtatg
      421 atggaaagaa gaacatttac actgtcacag cactgcccat tggcaacgaa cgggtcgact
      481 ttgaggtgac aatccctggg gaagggaagg atcgaatctt taaggtctcc atcaagtggc
      541 tagccattgt gagctggcga atgctgcatg aggccctggt cagcggccag atccctgttc
      601 ccttggagtc tgtgcaagcc ctggatgtgg ccatgaggca cctggcatcc atgaggtaca
      661 cccctgtggg ccgctccttc ttctcaccgc ctgagggcta ctaccacccg ctggggggtg
      721 ggcgcgaggt ctggttcggc tttcaccagt ctgtgcgccc tgccatgtgg aagatgatgc
      781 tcaacattga tgtctcagcc actgcctttt ataaggcaca gccagtgatt gagttcatgt
      841 gtgaggtgct ggacatcagg aacatagatg agcagcccaa gcccctcacg gactctcagc
      901 gcgttcgctt cacc
//
