LOCUS       sequence-7697-ex        2419 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Fehr M, Lalonde S, Lager I, Wolff MW, Frommer WB
  TITLE     In vivo imaging of the dynamics of glucose uptake in the cytosol of 
            COS-7 cells by fluorescent nanosensors.
  JOURNAL   J Biol Chem. 2003 May 23. 278(21):19127-33.
  PUBMED    12649277
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 May 23. 278(21):19127-33."
COMMENT     Sequence Label: pcDNA3.1 FLIPglu-600u
FEATURES             Location/Qualifiers
     source          1..2419
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(14..16,17..19,20..730)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1694..1696,1697..1699,1700..2413)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 ggatccgggc cgcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
       61 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      121 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      181 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc
      241 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      301 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      361 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      421 catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga
      481 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag
      541 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      601 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
      661 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
      721 gctgtacaag ggtggtaccg gaggcgccgc tgatactcgc attggtgtaa caatctataa
      781 gtacgacgat aacgcgatgt ctgtagtgcg caaggctatt gagcaagatg cgaaagccgc
      841 gccagatgtt cagctgctga tgaatgattc tcagaatgac cagtccaagc agaacgatca
      901 gatcgacgta ttgctggcga aaggggtgaa ggcactggca atcaacctgg ttgacccggc
      961 agctgcgggt acggtgattg agaaagcgcg tgggcaaaac gtgccggtgg ttttcttcaa
     1021 caaagaaccg tctcgtaagg cgctggatag ctacgacaaa gcctactacg ttggcactga
     1081 ctccaaagag tccggcatta ttcaaggcga tttgattgct aaacactggg cggcgaatca
     1141 gggttgggat ctgaacaaag acggtcagat tcagttcgta ctgctgaaag gtgaaccggg
     1201 ccatccggat gcagaagcac gtaccactta cgtgattaaa gaattgaacg ataaaggcat
     1261 caaaactgaa cagttacagt tagataccgc aatgtgggac accgctcagg cgaaagataa
     1321 gatggacgcc tggctgtctg gcccgaacgc caacaaaatc gaagtggtta tcgccaacaa
     1381 cgatgcgatg gcaatgggcg cggttgaagc gctgaaagca cacaacaagt ccagcattcc
     1441 ggtgtttggc gtcgatgcgc tgccagaagc gctggcgctg gtgaaatccg gtgcactggc
     1501 gggcaccgta ctgaacgatg ctaacaacca ggcgaaagcg acctttgatc tggcgaaaaa
     1561 cctggccgat ggtaaaggtg cggctgatgg caccaactgg aaaatcgaca acaaagtggt
     1621 ccgcgtacct tatgttggcg tagataaaga caacctggct gaattcagca agaaaggcgc
     1681 cggtaccggt ggaatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1741 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     1801 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1861 gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc
     1921 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1981 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     2041 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2101 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     2161 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     2221 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2281 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
     2341 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     2401 gctgtacaag taaaagctt
//
