LOCUS       sequence-76985-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ATG8 fused at the N terminus to GFP under the control of 
            the CUP1 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Wang K, Yang Z, Liu X, Mao K, Nair U, Klionsky DJ
  TITLE     Phosphatidylinositol 4-kinases are required for autophagic membrane 
            trafficking.
  JOURNAL   J Biol Chem. 2012 Nov 2;287(45):37964-72. doi: 
            10.1074/jbc.M112.371591. Epub 2012 Sep 13.
  PUBMED    22977244
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.371591"; journalName: "J Biol Chem"; date: 
            "2012-11-2- 2"; volume: "287"; issue: "45"; pages: "37964-72"
COMMENT     Sequence Label: CuGFP-ATG8(416)
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(444..464)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gactcactat agggcgaatt gggtaccggg ccccccctcg agactacctg ccaaatgtat
       61 tttctcctga gtaagtgaca tacaaaaacc cgtccttatc cttgtgttct tgatatatgg
      121 cagacatcaa cgccgcagta ggtggcaaag tatcattgac aaaaatgaag atggccttct
      181 cagggggtag cataattctc tttcttataa cataaacaaa ttgccctacg gtaaggtcag
      241 caggaactag atatttacgc ttatcaatct ctggaatatc tgacttttca gctttttcgc
      301 aaatcacagg tatcctattc ttgaacctgt cagcaatcct ctccgactcc gccttccttt
      361 tttcaaatgg atattcagac ttaaatgtag accccggggg atccttgtat agttcatcca
      421 tgccatgtgt aatcccagca gctgttacaa actcaagaag gaccatgtgg tctctctttt
      481 cgttgggatc tttcgaaagg gcagattgtg tggacaggta atggttgtct ggtaaaagga
      541 cagggccatc gccaattgga gtattttgtt gataatggtc tgctagttga acgcttccat
      601 cttcaatgtt gtgtctaatt ttgaagttaa ctttgattcc attcttttgt ttgtctgcca
      661 tgatgtatac attgtgtgag ttatagttgt attccaattt gtgtccaaga atgtttccat
      721 cttctttaaa atcaatacct tttaactcga ttctattaac aagggtatca ccttcaaact
      781 tgacttcagc acgtgtcttg tagttcccgt catctttgaa aaa
//
