LOCUS       sequence-76987-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Rodrigues MA, Gomes DA, Leite MF, Grant W, Zhang L, Lam W, Cheng YC,
            Bennett AM, Nathanson MH
  TITLE     Nucleoplasmic calcium is required for cell proliferation.
  JOURNAL   J Biol Chem. 2007 Jun 8. 282(23):17061-8.
  PUBMED    17420246
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Jun 8. 282(23):17061-8."
COMMENT     Sequence Label: pcDNA5-TO-PV-NES-DsRed
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..712)
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(97..120)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     512..532
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 gatcctcttc tgagatgagt ttttgttctg cggccgccag gaacaggtgg tggcggccct
       61 cggtgcgctc gtactgctcc acgatggtgt agtcctcgtt gtgggaggtg atgtccagct
      121 tggcgtccac gtagtagtag ccgggcagct gcacgggctt cttggccatg tagatggact
      181 tgaactccac caggtagtgg ccgccgtcct tcagcttcag ggccttgtgg gtctcgccct
      241 tcagcacgcc gtcgcggggg tacaggcgct cggtggaggc ctcccagccc atggtcttct
      301 tctgcatcac ggggccgtcg gaggggaagt tcacgccgat gaacttcacc ttgtagatga
      361 agcagccgtc ctgcagggag gagtcctggg tcacggtcgc cacgccgccg tcctcgaagt
      421 tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcttgtag tcggggatgt
      481 cggcggggtg cttcacgtac accttggagc cgtactggaa ctggggggac aggatgtccc
      541 aggcgaaggg cagggggccg cccttggtca ccttcagctt cacggtgttg tggccctcgt
      601 aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gtgccctcca
      661 tgcgcacctt gaagcgcatg aactcggtga tgacgttctc ggaggaggcc attgcggccg
      721 cgctttcggc caccagagtg gagaattctt caaccccaat cttgccgtcc ccgtccttgt
      781 ctccagcagc catcagcgtc tttgtttcct
//
