LOCUS       sequence-76991-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat16
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    51..75
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             90..112
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             123..167
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             183..200
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga
       61 gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata
      121 tgaaagaaac cgctgctgct aaattcgaac gccagcacat ggacagccca gatctgggta
      181 ccctggtgcc acgcggttcc atggcgatat cggatcggga aggtgtcaat gataatgaag
      241 aaggcttttt ctccgctcgt gaagaaatcc tgaatgctgt gtcgctggcg tgggtcgatg
      301 gtcaatccct gcgtattctg gaagttagtc tggatcgcgc atccgctcag ctggaaacca
      361 tcaaagaaga aaacccgttt ctgggctggc gtgttggtga cacggtcatt gaattcgatc
      421 tgccgctgct ggaagaaaag ctgtcaggct cggttaccgt cggtgaaacg ccggtgatcc
      481 gtgatgacga tccgtctttt gatgaactgg tggcgctggc agttgaaacc ggtcgtacgc
      541 aagttaacaa tgcggtgtca gttgacgaaa aactgattga ctcgcaggat gtggaaaccc
      601 gtggtctgga tgcgatcctg gttccgggcg gttttggcta tcgcgccatt ggtatcaatt
      661 tcattgatac ctacatccgt gcaggcgctt ggcaggtcat taactatcgc gaactggcaa
      721 atgtgcagga tctgaccgtc cgtgtgattg aactgcaagg tatcgccggt acgagtgcag
      781 cacgcattgt ggaaatctac ggtccggaaa gctctggcaa agcgggtgcc tg
//
